Similar Datasets
Project description:Transcriptome regulated by arterial aging in mice
| PRJNA608897 | ENA
Project description:This project aims to identify DNA methylation changes that occur during liver aging and regeneration using a whole-genome single nucleotide resolution approach and determine the genes and pathways associated with the differentially methylated genomic regions (DMRs) that are co-regulated by aging and regeneration
2023-01-28 | GSE211999 | GEO
Project description:Transcriptional profiling of mouse aortas comparing control wild-type animals with aortas from three different Plk1(+/-) mice. Goal was to determine the effects of Plk1 haploinsufficiency in aorta expression profiles.
2017-07-01 | GSE85199 | GEO
Project description:The impact of vascular aging (VA) has been extensively studied, yet little is known regarding the cellular and molecular mechanism underlying age-related VA in aortic cellular subpopulations. Herein, we analyzed transcriptomes and transposase-accessible chromatin profiles from aortas of 4-, 26-, and 86-week-old mice using single-cell RNA sequencing and ATAC sequencing. By integrating the heterogeneous transcriptome and chromatin accessibility data, we identified cell-specific transcription factor (TF) regulatory networks and open chromatin states. Moreover, we found that aortic aging affects cell interactions, inflammation, cell type composition and dysregulation of transcriptional control. Endothelial cell (EC) 1 has higher gene set activity related to senescence, aging, and adipogenesis than EC 2. Construction of senescence trajectories showed that the senescence of EC 1 and fibroblasts is associated with distinct TF open chromatin states and an mRNA expression model. Our data provide a system-wide model for transcriptional and epigenetic regulation during aortic aging at single-cell resolution.
2022-02-15 | GSE164585 | GEO
Project description:To determine whether an accelerated aging-like phenotype occurs in hematopoiesis of young Tif1γ-/- mice (4 months old), we purified 200,000 hematopoietic stem cells (LSK: Lineage negative, Sca1+, c-Kit+) from Tif1γ-/- mice and performed high-throughput mRNA sequencing (RNA-seq). We compared this transcriptome to physiological aging by creating two other RNAseq libraries from young (4 months old) and old (20 months old) wild type mice. RNAseq study on young Tif1γ-/- mice (4 months old), young wild type mice (4 months old) and old wild type mice (20 months old).
2014-06-16 | E-GEOD-57396 | biostudies-arrayexpress
Project description:To determine whether an accelerated aging-like phenotype occurs in hematopoiesis of young Tif1γ-/- mice (4 months old), we purified 200,000 hematopoietic stem cells (LSK: Lineage negative, Sca1+, c-Kit+) from Tif1γ-/- mice and performed high-throughput mRNA sequencing (RNA-seq). We compared this transcriptome to physiological aging by creating two other RNAseq libraries from young (4 months old) and old (20 months old) wild type mice.
2014-06-16 | GSE57396 | GEO
Project description:Aging increases the vulnerability to various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a nature product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate targets were identified and quercetin was further investigated due to its leading effects. Mechanistic studies revealed that in WS hMSCs, quercetin alleviated senescence via the enhancement of cell proliferation and the restoration and differentiation of heterochromatin architecture. RNA-seq analysis uncovered that quercetin and Vitamin C exerted geroprotective effects through different mechanisms. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS), physiological-aging and replicative-senescent hMSCs. More importantly, quercetin significantly improved the exercise endurance of old C57Bl/6 mice. Taken together, our study identifies quercetin as a geroprotective agent against premature and physiological aging, providing a novel therapeutic intervention for treating premature aging and promoting healthy aging.
2018-06-23 | GSE116166 | GEO
Project description:Background: miRNAs derived from peripheral venous blood gained extensive attention as clinical biomarkers, while arterial miRNAs exhibited slightly different expression profiles. We compared the expression profiles of venous- and arterial-derived plasma miRNA between young and aged male SD rats by next-generation sequencing, in order to explore whether peripheral venous miRNAs can represent entire circulating vessels in abnormal conditions like aging. Results: MSigDB Hallmark Gene Set reference and TAM 2.0 server were used to investigate the enriched functions and associated diseases. The aging-related de-regulated miRNAs in artery and vein shown similar enriched functional terms. Of note, refer to the arterial-versus-venous differential-expressed miRNA profiles, only a few miRNAs shared between young and aged rats. Among them, miR-450a/b and miR-223 shown the similar tendency between young and aged rat, while miR-136 and miR-503 etc displayed the opposite direction under the same scenario. Since miRNAs are under the control of their specific transcriptional factors, we further analyzed upstream regulators which influence miRNAs level for vascular vessel location. TransmiR v2.0 tool was used and found enriched upstream transcription factors like NFκB and SIRT1. These transcriptional factors could be organ-specific expression and/or regulated in physiological and aging states as parts of plausible causal factors. Conclusion: This study screened and analyzed the differential differential-expressed miRNA profiles in arterial and venous plasma under aging conditions, suggesting the importance of origin of candidate circulating miRNA biomarkers upon the certain scenario and its potential regulatory rule.
2019-10-03 | GSE138317 | GEO
Project description:The goal of this study is to identify aging-related biomarkers to improve our understanding of complex physiological changes, thereby providing a means to investigate the mechanism by which aging influences various diseases. Global microRNA expression patterns from peripheral blood of two groups of healthy young adult women, among which 13 were aged 22-25 and 9 were aged 36-39 years old, were compared to determine changes in microRNA expression that are associated with aging.
2013-08-13 | E-GEOD-49837 | biostudies-arrayexpress
Project description:The goal of this study is to identify aging-related biomarkers to improve our understanding of complex physiological changes, thereby providing a means to investigate the mechanism by which aging influences various diseases. Global gene expression patterns from peripheral blood of two groups of healthy young adult women, among which 13 were aged 22-25 and 9 were aged 36-39 years old, were compared to determine changes in gene expression that are associated with aging.
2013-08-13 | E-GEOD-49839 | biostudies-arrayexpress