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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [RNA-Seq]

ABSTRACT: Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of the human endometrium. Clinical application of eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro. A83-01, a selective TGFbeta receptor inhibitor, promotes pharmacological expansion of eMSC in culture by blocking differentiation and senescence. We subjected primary eMSC treated with A83-01 to RNA sequencing (RNA-seq) to map the underlying gene expression changes. RNA-seq analysis demonstrated that sustained TGFbeta-R inhibition results in differential expression of 1,463 genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE146066 | GEO | 2020/09/23

REPOSITORIES: GEO

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Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [ATAC-Seq]

Project description:Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of the human endometrium. Clinical application of eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro. A83-01, a selective TGFbeta receptor inhibitor, promotes pharmacological expansion of eMSC in culture by blocking differentiation and senescence. We subjected primary eMSC treated with A83-01 to Assay for Transposase Accessible Chromatin with sequencing (ATAC-seq) to map the underlying chromatin changes. A total of 185,084 open DNA loci were mapped accurately in EnSCs. ATAC-seq analysis demonstrated that sustained TGFbeta-R inhibition results in opening and closure of 3,555 and 2,412 chromatin loci, respectively. Motif analysis revealed marked enrichment of retinoic acid receptor (RAR) binding sites, which was paralleled by the induction of RARB.

2020-09-23 | GSE146065 | GEO

The transcriptome of human endometrial mesenchymal stem cells under TGFβR inhibition reveals improved potential for cell-based therapies

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2018-12-19 | GSE115137 | GEO

ISRIB can temporarily maintain the cell fate of human trophoblast stem cell without activating WNT and inhibiting TGFβ

Project description:To investigate the role of ISRIB in human trophoblast stem cell(hTSC) maintenance without activation of WNT & TGFβ. We performed gene expression profiling analysis using data obtained from RNA-seq of hTSCs cultured in the medium contained 0.5 µM ISRIB and 10 µM Y27652, after removing CHIR99021, A83-01, SB431542.

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Analysis of transcriptomes during cardioac reprogramming by RNA-deep Sequencing

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2019-09-01 | GSE135011 | GEO

Impacts of reprogramming method and culture conditions in trophoblast differentiation of induced pluripotent stem cells

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2015-08-31 | E-GEOD-64272 | biostudies-arrayexpress

Analysis of transcriptomes during cardioac reprogramming by RNA-deep Sequencing

2015-07-28 | GSE71405 | GEO

Small molecule-mediated reprogramming of human hepatocytes into bipotent progenitor cells

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2019-05-08 | GSE114643 | GEO

Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-23 | GSE146067 | GEO

Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model [RNA-seq]

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