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Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13

ABSTRACT: The 5th and 6th leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 and the empty vector control line FOX3 were examined. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 (OsLBD37) ortholog of Arabidopsis in nitrogen metabolism- and senescence- related processes.

ORGANISM(S): Oryza sativa

PROVIDER: GSE14647 | GEO | 2009/12/27

SECONDARY ACCESSION(S): PRJNA114435

REPOSITORIES: GEO

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Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13

Project description:The 5th and 6th leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 and the empty vector control line FOX3 were examined. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 (OsLBD37) ortholog of Arabidopsis in nitrogen metabolism- and senescence- related processes. Hygromycin- resistant rice Os-LBD37 overexpressor and empty vector control plants were grown in hydroponic culture system. The sampling date of the leaf blades of RK16331-13 LBD37 overexpressor plants and the empty vector control was determined according to the emergence of the 8th leaf blade. Three independent hybridizations were performed for line RK16331-13 and the empty vector control line.

2009-12-27 | E-GEOD-14647 | biostudies-arrayexpress

Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants

2009-12-27 | GSE14646 | GEO

Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants

Project description:Aerial parts of the rice-Arabidopsis FOX (Full-length cDNA overexpressor) lines K16331 and K19624 harboring the rice FL cDNA of LBD37 (Os-LBD37) were analyzed. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 ortholog of Arabidopsis in nitrogen metabolism- related processes. K16331 (T2) and empty vector control plants (T2) were grown for 18 days on solid MS- medium supplemented with 20mg/l Hygromycin. For each hybridization on Affymetrix microarrays, 2 independent plants for K16331 were used. Homozygote K19624 (T3) and empty vector control plants (T3) were grown for 18 days on solid MS- medium. For each hybridization on Affymetrix microarrays, 1 plant was used.

2009-12-26 | E-GEOD-14646 | biostudies-arrayexpress

Involvement of the rice LBD37 (OsLBD37) ortholog of Arabidopsis in nitrogen metabolism- and senescence-related processes

Project description:This SuperSeries is composed of the following subset Series: GSE14646: Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants GSE14647: Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 Refer to individual Series

2009-12-26 | E-GEOD-14663 | biostudies-arrayexpress

Transcriptome analysis of Arabidopsis VIRE2-INTERACTING PROTEIN2 Overexpressor in Agrobacterium-mediated plant transformation and abiotic stresses

Project description:VIP2 is a transcription regulator and the C-terminal NOT2 domain of VIP2 interacts with VirE2. However, AtVIP2 overexpressor lines in Arabidopsis did not show an improvement in Agrobacterium-mediated stable root transformation. To investigate the role of VIP2 in Agrobacterium-plant interaction, we carried out transcriptome profiling by comparing a homozygous Arabidopsis AtVIP2 overexpressor line with wild-type Col-0 plants following Agrobacterium infection.

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Transcriptome analysis of genes regulated by overexpression of LATERAL ORGAN BOUNDARIES (LOB) in Arabidopsis thaliana

Project description:The Arabidopsis thaliana transcription factor LATERAL ORGAN BOUNDARIES (LOB) is expressed in the boundary between the shoot apical meristem and initiating lateral organs. To identify genes regulated by LOB activity, we used an inducible 35S:LOB-GR line. This analysis identified genes that are differentially expressed in response to ectopic LOB activity.

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STOP1 overexpressor vs. Col-0 and stop1 knockout vs Col-0 under control and NaCl

Project description:Transcriptional profiling of Arabidopsis thaliana seedlings comparing overexpressor with wild type, and knockout with wild type separately under control and NaCl stress.

2018-03-01 | GSE102948 | GEO

Oryza sativa

Project description:Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13

| PRJNA114435 | ENA

Leishmania major: Sir2rp3 overexpressor vs wild-type

Project description:Transcriptional profiling of Leishmania major parasites overexpressing LmSir2rp3 protein compared with wild-type cell line transfected with empty vector in normal growth conditions. Goal of this experiment was to evaluate the possible effect of LmSir2rp3 in the control of gene expression in this protozoan parasite that has a polycistronic transcription. Two-condition experiment, LmSir2rp3 overexpressor vs. Wild-type cell line bearing empty vector. Biological replicates, 3 LmSir2rp3 overexpressor replicates and 3 wild-type cell line bearing empty-vector

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Transcriptome analysis of genes regulated by overexpression of LATERAL ORGAN BOUNDARIES (LOB) in Arabidopsis thaliana

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