Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance

ABSTRACT: Background: Brain glucose-sensing neurons detect glucose fluctuations and prevent severe hypoglycemia, but mechanisms mediating functions of these glucose-sensing neurons are unclear. Methods: We combined mouse genetics, electrophysiology, neural tracing, optogenetics and Patch-RNA-seq to demonstrate how glucose-sensing neurons in the ventrolateral VMH regulate glucose balance. Results: Here we report that estrogen receptor-α (ERα)-expressing neurons in the ventrolateral subdivision of the ventromedial hypothalamic nucleus (vlVMH) are glucose-sensing neurons to a 5-1-5mM glucose fluctuation, being glucose-inhibited neurons (GI-ERαvlVMH) or glucose-excited neurons (GE-ERαvlVMH). Hypoglycemia activates GI-ERαvlVMH neurons via the anoctamin 4 channel, and inhibits GE-ERαvlVMH neurons through opening the ATP-sensitive potassium channel. Further, we show that GI-ERαvlVMH neurons preferentially project to the medioposterior arcuate nucleus of the hypothalamus (mpARH) and GE-ERαvlVMH neurons preferentially project to the dorsal Raphe nuclei (DRN). Activation of ERαvlVMH-mpARH circuit and inhibition of ERαvlVMH-DRN circuit both increase blood glucose. Conclusions: Our results indicate that ERαvlVMH neurons detect glucose fluctuations and prevent severe hypoglycemia in mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE146543 | GEO | 2020/04/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

TRAP experiment to determine gene expression changes in absence of AMPK activity in the VMN

Project description:The ventromedial hypothalamus (VMN) is implicated in the regulation of many aspects of energy homeostasis, including the counterregulatory response to hypoglycemia. Neurons of the VMN that are activated by hypoglycemia (glucose inhibited neurons (GI)) have been assumed to play a critical, although untested role in this response. Here, we used that expression of a dominant negative form of AMP-activated protein kinase (AMPK) in Sf1 neurons of the VMN which selectively suppressed GI neurons activity. We then performed Translating Ribosome Affinity Purification (TRAP Seq) to evaluate the transcriptional changes induced specifically in that cell population.

2020-10-28 | GSE153872 | GEO

RNA-seq Identification of Candidate Genes Differentially Expressed in DRN-BA(Pyr) and DRN-BA(PV) Neurons

Project description:To investigate the diversity of BA-projecting DRN(5-HT) neurons, we performed RNA-seq of BA(Pyr)- and BA(PV)-projecting DRN(5-HT) neurons and compared their gene expression levels.

2022-12-31 | GSE214895 | GEO

Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance

Project description:Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance

| PRJNA610836 | ENA

Molecular and anatomical organization of the dorsal raphe nucleus

Project description:The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection the DRN and its diverse functions.

2019-08-06 | GSE134163 | GEO

Repeated hypoglycemia in diabetic mice induce hypothalamic mitochondrial and synaptic activity deregulations characteristic of neurodegenerative diseases

Project description:Repeated exposures to insulin-induced hypoglycemia in diabetic patients progressively impair the counter-regulatory response (CRR) that restores normoglycemia. This defect is characterized by reduced secretion of glucagon and other counter-regulatory hormones. Evidence indicates that glucose responsive neurons located in the hypothalamus, orchestrate the CRR. Here, we aimed at identifying the changes in hypothalamic gene and protein expression that underlie impaired CRR. High fat diet fed and low dose streptozocin-treated C57BL6N mice were exposed to one (acute hypoglycemia, AH) or multiple (recurrent hypoglycemia, RH) insulin-induced hypoglycemic episodes. Single nuclei RNA sequencing (snRNAseq) data were obtained from the hypothalamus and cortex of AH and RH mice. Proteomic data were also obtained from hypothalamic synaptosomal fractions. The present study shows that repeated moderate hypoglycemia in diabetic mice lead, in the hypothalamus, to multiple cellular physiology changes, affecting all cell types and their interactions, which show striking features of neurodegenerative diseases. It also shows that repeated hypoglycemic episodes affect very differently the hypothalamus and the cortex.

2023-11-13 | PXD040183 | Pride

Gene expression profile at single nuclei level of brain tissues from diabetic mice exposed to acute or recurrent hypoglycemia.

Project description:The brain is an important controller of glucose homeostasis and mediates counterregulatory responses to hypoglycemia. We used single-nuclei transcriptomics to study the mechanisms of defective hypoglycaemia counter-regulation in brain in a model of diabetic mice exposed to acute or recurrent hypoglycemia.

2023-10-02 | GSE226277 | GEO

Increased homing of immune cells by orexigenic catecholaminergic neurons alleviates autoimmune disorder

Project description:Whole-body energy status significantly impacts the immune system. Both feeding behavior and energy metabolism are controlled by the central nerve system, but it is unknown whether the brain can regulate the immune system in response to fluctuations of the energy status. Here, we show that catecholaminergic (CA) neurons in the nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM) were activated by fasting, and demonstrate that chemogenetic activation of these hindbrain CA neurons both increased food intake and drove homing of CD45+ cells, T cells, and B cells from blood to bone marrow, which was mediated by enhanced HPA axis and upregulation of the chemokine receptor CXCR4. This activation of CA neurons also prevented immune cell infiltration to the brain in the experimental autoimmune encephalomyelitis (EAE) mouse model, and ameliorated the disease outcome. Our study demonstrates the neuronal control of immune function in response to host energy scarcity and sheds light on communications between the nervous and immune systems.

2024-03-01 | GSE170737 | GEO

drn/drn-l expressing cells vs non-expressing cells-Embryonic patterning in Arabidopsis thaliana

Project description:adt05-04_drn - drn/drn-l expressing cells vs non-expressing cells - DRN targets - In primordia timing (pt) mutant background DRN::erGFP transgenic cell culture (somatic embryos suitable to regenerate plants) has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells. Total RNA was extracted and sent to Evry for comparison (samples DRN a,b,c). DRN-L::erGFP transgenic cell culture has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells (samples DRN-L-2-1 and DRN-L-3-1). Keywords: null 5 dye-swap - CATMA arrays

2010-04-30 | E-GEOD-19799 | biostudies-arrayexpress

drn/drn-l expressing cells vs non-expressing cells-Embryonic patterning in Arabidopsis thaliana

2010-05-01 | GSE19799 | GEO

RNA-Seq analysis of galaninergic neurons from ventrolateral preoptic nuleus identifies expression changes between sleep and wake

Project description:Galanin neurons at ventrolateral preoptic nuleus (VLPO) have been hypothesized to be sleep-active neurons. With the use of laser capture microdissection (LCM), we specifically isolated single galanin neurons, and our goal was to assess expression changes in VLPO galanin cells between sleeping mice and mice kept awake using gentle handling.

2020-08-27 | GSE132308 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data