Temporal regulatory role of miR156 during leaf development
Ontology highlight
ABSTRACT:
ORGANISM(S): Oryza sativa Oryza sativa Japonica Group
PROVIDER: GSE14692 | GEO | 2010/10/26
SECONDARY ACCESSION(S): PRJNA111685
REPOSITORIES: GEO
Similar Datasets
Project description:Analysis of different leaves from transgenic plants over expressing microRNA miR156. Time-dependent gene expression is essential for developmental timing control. MiR156 was characterized as a time-dependent expressed microRNA during leaf development. The level of miR156 is positive correlated to developmental time during leaf growth. We used microarray to identify genes regulated by miR156 in a time-dependent manner during leaf development. Results indicate that most time-negative genes were activated in mature leaves of miR156 over expressed plants compared with wild type. Keywords: gene expression array-based (RNA / in situ oligonucleotide) To identify the temporal regulation role of miR156 in different leaves, all leaves in the main tiller were collected separately from Md/Mh (miR156 over expressed lines) and wild type (WT) plants. The level of miR156 were checked in these samples, then three biological repeats were selected and these RNA samples of the oldest (L1) and youngest leaf (L4 or L5) were applied for microarray hybridization.
2010-10-26 | E-GEOD-14692 | biostudies-arrayexpress
Project description:Background: The change from juvenile to mature phase in woody plants is often accompanied by a gradual loss of rooting ability, as well as by reduced microRNA (miR) 156 and increased miR172 expression. Results: We characterized the population of miRNAs of Eucalyptus grandis and compared by Northern blot the gradual reduction in miR156 and increase in miR172 expression during development to the loss of rooting ability. Forty known and eight novel miRNAs were discovered and their predicted targets are listed. The expression pattern of nine miRNAs was determined during adventitious root formation in juvenile and mature cuttings. While the expression levels of miR156 and miR172 were inverse in juvenile and mature tissues, no mutual relationship was found between high miR156 expression and rooting ability, or high miR172 expression and loss of rooting ability. This is shown both in E. grandis and also in E. brachyphylla, in which explants that underwent rejuvenation in tissue culture conditions were also examined. Conclusions: It is suggested that in these Eucalyptus species, there is no correlation between the switch of miR156 with miR172 expression in the stems and the loss of rooting ability. Examination of microRNA in seedlings of Eucalyptus grandis
2014-10-01 | E-GEOD-58367 | biostudies-arrayexpress
Project description:Heat stress and extreme temperatures negatively affect plant development by disrupting regular cellular and biochemical functions, ultimately leading to reduced crop production. Recently, our group has shown through physiological experiments that miR156 overexpression resulted in an improved alfalfa response to heat stress. To further expand the scope of miR156 research, we employed a label-free quantification based quantitative proteomics approach to explore the effects of heat stress on protein levels in miR156OE alfalfa. Our major objective was to identify miR156-regulated gene products with differentially altered abundance under heat stress in alfalfa.
2020-10-16 | PXD019560 | Pride
Project description:Background: The change from juvenile to mature phase in woody plants is often accompanied by a gradual loss of rooting ability, as well as by reduced microRNA (miR) 156 and increased miR172 expression. Results: We characterized the population of miRNAs of Eucalyptus grandis and compared by Northern blot the gradual reduction in miR156 and increase in miR172 expression during development to the loss of rooting ability. Forty known and eight novel miRNAs were discovered and their predicted targets are listed. The expression pattern of nine miRNAs was determined during adventitious root formation in juvenile and mature cuttings. While the expression levels of miR156 and miR172 were inverse in juvenile and mature tissues, no mutual relationship was found between high miR156 expression and rooting ability, or high miR172 expression and loss of rooting ability. This is shown both in E. grandis and also in E. brachyphylla, in which explants that underwent rejuvenation in tissue culture conditions were also examined. Conclusions: It is suggested that in these Eucalyptus species, there is no correlation between the switch of miR156 with miR172 expression in the stems and the loss of rooting ability.
2014-10-01 | GSE58367 | GEO
Project description:The widespread agricultural problem of pre-harvest sprouting (PHS) could potentially be overcome by improving seed dormancy. Here, we report that miR156, an important grain yield regulator, also controls seed dormancy in rice. We found that mutations in one MIR156 subfamily enhance seed dormancy and suppress PHS with negligible effects on shoot architecture and grain size, whereas mutations in another MIR156 subfamily modify shoot architecture and increase grain size but have minimal effects on seed dormancy. Mechanistically, mir156 mutations enhance seed dormancy by suppressing the gibberellin (GA) pathway through de-represssion of the miR156 target gene Ideal Plant Architecture 1 (IPA1), which directly regulates multiple genes in the GA pathway. These results provide an effective method to suppress PHS without compromising productivity, and will facilitate breeding elite crop varieties with ideal plant architectures.
2019-09-03 | GSE131243 | GEO
Project description:In plants, micro RNAs and chromatin remodeling are both known to regulate plant development and reproduction, however evidences for molecular links between these pathways have remained sparse. The antagonistic activities of miR156 and its target mRNAs, encoding several SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, control the juvenile-to-adult and vegetative-to-reproductive developmental phase transitions. In recent years a number of proteins were shown to act in the transcriptional control of MIR156 genes, demonstrating the complexity of the regulatory network involved in this process. Spatiotemporal control of gene expression involves interactions between transcription factors and changes in the chromatin structure. SPLAYED (SYD) is a well-characterized SWI2/SNF2 chromatin remodeling ATPase, which has been shown to be an important regulator of flowering time, flower organ identity and homeotic gene expression. In this study, we investigated the interaction between the miR156 and SYD pathways and provide evidence that SYD directly influences the transcription of several MIR156 and SPL genes in Arabidopsis. We propose that SYD maintains the balance between the miR156-dependent and miR156-independent developmental pathways. More generally, we then show that SYD may also play a very broad role in the control of gene expression by associating to most genes in Arabidopsis. We show that binding to these genes is dependent on the meristem identity factor LEAFY and the PRC2 component SWINGER. These results uncover a much more general role for SYD in regulating and balancing key developmental processes in Arabidopsis than previously assumed.
2020-10-12 | GSE104665 | GEO
Project description:Rice leaves consist of three distinct regions along a proximal-distal axis, namely, the leaf blade, sheath, and blade-sheath boundary region. Each region has a unique morphology and function, however,but the genetic programs underlying the development of each region are poorly understood. To capture the entire picture of rice leaf development and to discover genes with unique functions in rice and grasses, it is crucial to explore genome-wide transcriptional profiles during the development of the three regions. In this study, we performed microarray analysis to profile the spatial and temporal patterns of gene expression in the rice leaf using dissected parts of leaves sampled in broad developmental stages.
2020-10-06 | GSE159047 | GEO
Project description:Early leaf growth is sustained by cell proliferation and subsequent cell expansion that initiates at the leaf tip and proceeds in a basipetal direction. Using detailed kinematic and gene expression studies to map these stages during early development of the third leaf of Arabidopsis thaliana we showed that the cell cycle arrest front did not progress gradually down the leaf, but rather was established and abolished abruptly. Interestingly, leaf greening and stomatal patterning followed a similar basipetal pattern, but proliferative pavement cell and formative meristemoid divisions were uncoordinated in respect to onset and persistence. Genes differentially expressed during the transition from cell proliferation to expansion were enriched in genes involved in cell cycle, photosynthesis, and chloroplast retrograde signaling. Proliferating primordia treated with norflurazon, a chemical inhibitor of retrograde signaling, showed inhibited onset of cell expansion. Hence, differentiation of the photosynthetic machinery is important for regulating the exit from proliferation. GSM839070-GSM839081: Total RNA was extracted using RNeasy plant mini kit (Qiagen) from leaf 3 of Arabidopsis seedlings grown in vitro and treated or not treated with norflurazon (NF) at 8 days after stratification (DAS) and harvested 24 and 48 hours after treatment. RNA from three biological repeats of each timepoint and treatment were submitted to AGRONOMICS1 array hybridization. GSM839082-GSM839099: Total RNAs were extracted using RNeasy plant mini kit (Qiagen) from leaf 3 of Arabidopsis seedlings 8, 9, 10, 11, 12, and 13 days after stratification (DAS) and grown in vitro. RNAs from three biological repeats of each timepoint were submitted to AGRONOMICS1 array hybridization.
2012-01-17 | E-GEOD-33936 | biostudies-arrayexpress
Project description:Microarray analysis of wild type plants and plants with reduced (ago1-27 and se-1) or increased miR156 levels (se-1 p35S:MIR156). Shoot apices were dissected from 20-day-old, short-day grown plants.
2009-06-12 | GSE16061 | GEO
Project description:Early leaf growth is sustained by cell proliferation and subsequent cell expansion that initiates at the leaf tip and proceeds in a basipetal direction. Using detailed kinematic and gene expression studies to map these stages during early development of the third leaf of Arabidopsis thaliana we showed that the cell cycle arrest front did not progress gradually down the leaf, but rather was established and abolished abruptly. Interestingly, leaf greening and stomatal patterning followed a similar basipetal pattern, but proliferative pavement cell and formative meristemoid divisions were uncoordinated in respect to onset and persistence. Genes differentially expressed during the transition from cell proliferation to expansion were enriched in genes involved in cell cycle, photosynthesis, and chloroplast retrograde signaling. Proliferating primordia treated with norflurazon, a chemical inhibitor of retrograde signaling, showed inhibited onset of cell expansion. Hence, differentiation of the photosynthetic machinery is important for regulating the exit from proliferation.
2012-01-17 | GSE33936 | GEO