Identification of m6A residues at single nucleotide resolution using eCLIP and an accessible custom analysis pipeline

ABSTRACT: We have developed a modified eCLIP-based method (meCLIP) to identify m6A residues at single-nucleotide resolution. By coupling the improvements of eCLIP with an easy-to-use computational pipeline, we have successfully identified over 50,000 unique m6A residues in the two breast cancer cell lines that were analyzed (MCF-7 and MDA-MB-231) and over 8,000 unique residues in HEK-293 cells. We compared these residues to the sites called using the currently most utilized m6A identification method (miCLIP).

ORGANISM(S): Homo sapiens

PROVIDER: GSE147440 | GEO | 2020/12/31

REPOSITORIES: GEO