In situ HiC of primary mouse B cell progenitor populations after activation with LPS

ABSTRACT: Naive resting B-cells were purified from the bone marrow of C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS. Activated but undivided cells were harvested 3 hours, 10 hours or 33 hours after the LPS treatment. The Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) system was also used to isolate imminently dividing B cells specifically in the G1 stage of the cell cycle prior to the S phase.

ORGANISM(S): Mus musculus

PROVIDER: GSE147467 | GEO | 2020/12/08

REPOSITORIES: GEO