Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

ABSTRACT: To identify the genes that are regulated by IRF7, we have performed DNA microarray in 3T3-L1 adipocytes differentiated from precursor cells infected with retrovirus empty or carrying IRF7. Plasmids (Empty- and IRF7-pMSCV) were kindly provided from Dr. Eguchi at the Okayama University Graduate School of Medicine, Okayama, Japan [Cell Metab. 2008;7: 86-94.]. Infected 3T3-L1 preadipocytes were selected by puromycin treatment and differentiated into adipocytes. 7 days after the induction of adipogenesis, total RNA was isolated.

ORGANISM(S): Mus musculus

PROVIDER: GSE147857 | GEO | 2020/04/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

Project description:Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

| PRJNA622292 | ENA

Multiple Roles for Steroid Receptor RNA Activator (SRA) in Regulation of Adipogenesis and Insulin Sensitivity

Project description:PPARM-NM-3 is a master transcriptional regulator of adipogenesis. Hence, the identification of PPARM-NM-3 coactivators should help reveal mechanisms controlling gene expression in adipose tissue development and physiology. We show that the non-coding RNA Steroid receptor RNA Activator, SRA, associates with PPARM-NM-3 and coactivates PPARM-NM-3-dependent reporter gene expression. Overexpression of SRA in ST2 adipocyte precursor cells promotes their differentiation into adipocytes. Conversely, knockdown of endogenous SRA inhibits 3T3-L1 preadipocyte differentiation. Microarray analysis reveals hundreds of SRA-responsive genes in adipocytes, including genes in cell cycle, insulin and TNFM-NM-1 signaling pathways. Some functions of SRA may involve mechanisms other than coactivation of PPARM-NM-3. SRA increases insulin-stimulated glucose uptake in adipocytes. SRA promotes S-phase entry during mitotic clonal expansion, decreases expression of cyclin-dependent kinase inhibiters p21Cip1 and p27Kip1, and increases phosphorylation of Cdk1/Cdc2. SRA also inhibits the TNFM-NM-1-induced phosphorylation of c-Jun NH2-terminal kinase. In conclusion, SRA enhances adipogenesis and adipocyte function through multiple pathways. Total RNA was isolated from fully differentiated (MDIT day 4) SRA overexpressing (pMSCV-SRA) and control (pMSCV empty vector) ST2 adipocytes, or fully differentiated (MDIT day 8) shSRA knockdown (pSuperior-shSRA) or shControl (pSuperior-shcontrol) 3T3-L1 adipocytes. Genome wide gene expression analysis was performed using Affymetrix mouse genome 430 2.0 arrays. Triplicate samples were analyzed.

2013-05-07 | E-GEOD-21594 | biostudies-arrayexpress

MicroRNA profiling of mouse 3T3-L1 cells before and after adipogenesis

Project description:Compare miRNA expression profiles between undifferentiated 3T3-L1 preadipocytes (Day 0) and differentiated 3T3-L1 adipocytes (Day 9)

2009-02-19 | E-MEXP-1930 | biostudies-arrayexpress

RNAi knockdown of Tip60 and Usp7 in mouse 3T3-L1 adipocytes

Project description:Knockdown of Tip60 or Usp7 in differentiated 3T3-L1 adipocytes

2013-08-28 | E-TABM-1145 | biostudies-arrayexpress

Gene expression profiles of human breast cancer cell lines MCF-7, T47D and MDA-MB-231 co-cultured with 3T3-L1 adipocytes.

Project description:Obesity is a known risk factor for breast cancer. To identify genes and underlying pathways in human breast cancer cells affected by interaction with mature adipocytes, two estrogen-receptor positive (ER+) breast cancer cell lines, MCF-7 and T47D, and the triple-negative (TN) breast cancer cell line MDA-MB-231 were cultivated in a co-culture system with or without differentiated murine 3T3-L1 adipocytes for the purpose of a microarray gene expression analysis. The use of in vitro differentiated 3T3-L1 adipocytes allowed comparable experimental conditions for each of the co-culture experiments with human breast cancer cell lines. For co-cultivation analyses of 3T3-L1 and breast cancer cells, we set up a two-dimensional transwell system, which enables intercellular communication through soluble factors secreted into the medium but inhibits intermixture of the different cell types. Following 5 days of co-culture with or without differentiated adipocytes, total RNA was isolated from the human breast cancer cells and subjected to microarray gene expression analyses.

2018-06-22 | E-MTAB-6855 | biostudies-arrayexpress

Zinc Finger Protein 407 (ZFP407) Regulates Insulin-Stimulated Glucose Uptake and Glucose Transporter 4 (Glut4) mRNA

Project description:Using RNA-Seq, we compared the transcriptomes of differentiated 3T3-L1 adipocytes for control and ZFP407-deficient cells Differentiated 3T3-L1 cells were electroporated with control or 1 of 2 Zfp407 siRNAs. Six independent siRNA electroporations were conducted for the control siRNA and 3 independent electroporations were conducted for each Zfp407 siRNA.

2015-01-16 | E-GEOD-64757 | biostudies-arrayexpress

Zinc Finger Protein 407 (ZFP407) Regulates Insulin-Stimulated Glucose Uptake and Glucose Transporter 4 (Glut4) mRNA

Project description:Using RNA-Seq, we compared the transcriptomes of differentiated 3T3-L1 adipocytes for control and ZFP407-deficient cells

2015-01-16 | GSE64757 | GEO

Real-time quantitative PCR analysis of 3T3-L1 cells treated with Interleukin-15 (IL-15)

Project description:Differentiated 3T3-L1 adipocytes were either treated 0ng/ml or 500ng/ml rmIL-15 for 24 hours (n=3/group)

2014-11-14 | GSE63253 | GEO

The effects of adenine-midiated purinergic signaling in 3T3 fibroblasts and adipocytes

Project description:In previous study, we demonstrated that adenine could activate AMPK signaling in 3T3 fibroblast. To investigate the changes of transcriptome in differential 3T3 cells by adenine stimulation, we used microarrays to analyze undifferentited annd differentiated 3T3-L1 adipocytes in mRNA levels.

2017-02-07 | GSE94539 | GEO