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High-throughput RNA sequencing of basilar artery smooth muscle cells in wild type and high-salt diet induced hypertension rats

ABSTRACT: We utilized high-throughput sequencing and subsequent signaling pathway analyses to find 2 fold change or greater upregulated expression of 230 transcripts and downregulated expression of 165 transcripts in basilar artery smooth muscle cells derived from rats fed a high-salt diet compared with those from control rats.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE147945 | GEO | 2020/04/02

REPOSITORIES: GEO

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High-throughput RNA sequencing of basilar artery smooth muscle cells in wild type and high-salt diet induced hypertension rats

Project description:High-throughput RNA sequencing of basilar artery smooth muscle cells in wild type and high-salt diet induced hypertension rats

| PRJNA622585 | ENA

Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

Project description:New Zealand white rabbits underwent bilateral carotid ligation to increase flow in the basilar artery (BA) or sham surgery. 24 hours post-surgery, the BA and basilar terminus (BT) were embedded for sectioning. The intima and media were separately laser-microdissected from the sections, and whole transcriptome RNA-seq was performed on each tissue in order to more thoroughly characterize potential molecular signals between endothelium and smooth muscle during flow-induced arterial remodeling.

2021-07-28 | GSE169276 | GEO

Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

2016-06-30 | GSE61212 | GEO

Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

Project description:High-flow causes the remodeling of arteries, in which smooth muscle cells play an important role. To know the profile of smooth muscle gene expression under high-flow conditions in vivo, flow of rabbit basilar artery was increased by ligation of both common carotid arteries. Microarrays were performed to profile the gene expression of smooth muscle cells isolated from rabbit basilar artery. Expression profiles indicate 43603 differentially expressed genes in smooth muscle cells exposed to high-flow insult compared with the sham control, of which 1470 genes were upregulated and 780 genes downregulated using 2 fold-changes and P<0.05 as a cut-off. Bilateral common carotid arteries of female New Zealand White rabbits were ligated to increase vascular flow.The control group was performed the same procedure to expose the CCAs without ligation. Rabbits were euthanized at day 5 after ligation or exposure of bilateral CCAs in both groups (n=3 for each group). The rabbits used and all procedures in this study were approved by the local Institutional Animal Care and Use Committee. Smooth muscle cells were isolated. After euthanization of rabbits, the whole basilar arteries were removed. The arteries were cleaned in PBS buffer,cannulated and perfused at a constant flow with a cocktail which contains PBS and 0.4 mg/ml elastase (Sigma) and 1 mg/ml collagenase (type 1A, Sigma). After an incubation time of 45 min, the tissue left was removed and stored in PBS. SMCs were released from the artery by trituration. Then Total RNA was extracted and gene chip tests were performed.

2016-06-30 | E-GEOD-61212 | biostudies-arrayexpress

Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

Project description:Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

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Expression data from control and gentamicin-treated chick basilar papilla

Project description:In chick basilar papilla, hair cells can be regenerated after gentamicin treatment. To identify genes and pathways involved in this process, we performed microarray analysis on the basilar papilla 0, 48 and 72 hours after gentamicin.

2016-12-01 | GSE79963 | GEO

The Transcriptome of Basilar Papilla Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken basilar papilla hair cell across a 7 days regenerative time course. Compare gene expression changes in chicken basilar papilla treated with streptomycin to the control. A total of 60 samples were collected from 8 different time points. Each time point contains 2 biological samples.

2015-02-21 | E-GEOD-66101 | biostudies-arrayexpress

Transcription profiling by array of chicken basilar papillae from low, middle and high frequency segments of the auditory epithelia

Project description:Basilar papillae (i.e.auditory epithelia) were isolated from 4-day-old chickens and sectioned into low, middle, and high frequency segments. RNA was isolated from each segment separately, amplified using a two-cycle approach, biotinylated, and hybridized to Affymetrix chicken whole-genome arrays.

2010-08-13 | E-MEXP-2806 | biostudies-arrayexpress

The Transcriptome of Basilar Papilla Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken basilar papilla hair cell across a 7 days regenerative time course.

2015-02-21 | GSE66101 | GEO

Renal medullary genes in salt-sensitive hypertension

Project description:Substitution of chromosome 13 from Brown Norway BN/SsNHsd/Mcw (BN/Mcw) rats into the Dahl salt-sensitive SS/JrHsd/Mcw (SS/Mcw) rats resulted in substantial reduction of blood pressure salt sensitivity in this consomic rat strain designated SSBN13. In the present study, we attempted to identify genes associated with salt-sensitive hypertension by utilizing a custom, known-gene cDNA microarray to compare the mRNA expression profiles in the renal medulla (a tissue playing a pivotal role in long-term blood pressure regulation) of SS/Mcw and SSBN13 rats on either low-salt (0.4% NaCl) or high-salt (4% NaCl, 2 wk) diets. Keywords: Dahl S rat; blood pressure; kidney; consomic rats

2005-11-17 | GSE3608 | GEO

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