Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional analysis of isolated intestinal epithelial cells (IEC) from adult infected and age-matched control animals

ABSTRACT: G. lamblia is a fecal-oral transmitted human enteropathogenic protozoan with extremely high incidence in endemic areas in Africa and Asia particularly in the infant population. It homes to the proximal small intestine and induceses diarrhea and malabsoption. Here we established a novel murine G. lamblia infection model and analyzed the tissue and epithelial response and downstream microbial and metabolic effects in the adult host.

ORGANISM(S): Mus musculus

PROVIDER: GSE148081 | GEO | 2020/04/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [intestinal epithelial cells]

Project description:G. lamblia is a fecal-oral transmitted human enteropathogenic protozoan with extremely high incidence in endemic areas in Africa and Asia particularly in the infant population. It homes to the proximal small intestine and induced diarrhea and malabsoption. Here we established a novel murine G. lamblia infection model and analyzed the tissue response and downstream microbial and metabolic effects in the host.

2020-06-01 | GSE113667 | GEO

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [small intestinal tissue]

2020-06-01 | GSE113666 | GEO

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [liver]

2020-06-01 | GSE113679 | GEO

Transcriptional analysis of total small intestinal tissue from adult infected and age-matched control animals

Project description:G. lamblia is a fecal-oral transmitted human enteropathogenic protozoan with extremely high incidence in endemic areas in Africa and Asia particularly in the infant population. It homes to the proximal small intestine and induceses diarrhea and malabsoption. Here we established a novel murine G. lamblia infection model and analyzed the tissue and epithelial response and downstream microbial and metabolic effects in the adult host.

2020-04-03 | GSE148082 | GEO

Innate Lymphoid Cells-Containing Fraction of Murine Lamina Propria Cells Produces Interleukin-17 upon Incubation with Giardia lamblia

Project description:Innate lymphoid cells (ILCs) are key players during an immune response at the mucosal surfaces, such as lung, skin, and gastrointestinal tract. Giardia lamblia is an extracellular protozoan pathogen that inhabits the human small intestine. In this study, an ILC-containing fraction (ICF) were prepared from the lamina propria of murine small intestine and incubated with G. lamblia trophozoites in the presence of transwell membranes. Transcriptional changes in Giardia-exposed ICFs were examined by RNA-seq analysis, resulting in identification of activation of several immune pathways.

2019-04-25 | GSE130256 | GEO

Identification of cell cycle-related genes up-regulated during the G2-phase in Giardia lamblia

Project description:To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the two stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

2019-02-20 | GSE126731 | GEO

Population structure shapes copy number variation in malaria parasites

Project description:If copy number variants (CNVs) are predominantly deleterious, we would expect them to be more efficiently purged from populations with a large effective population size (Ne) than from populations with a small Ne. Malaria parasites (Plasmodium falciparum) provide an excellent organism to examine this prediction, because this protozoan shows a broad spectrum of population structures within a single species, with large, stable, outbred populations in Africa, small unstable inbred populations in South America and with intermediate population characteristics in South East Asia. We characterized 122 single-clone parasites, without prior laboratory culture, from malaria-infected patients in 7 countries in Africa, SE Asia and S. America using a high density SNP/CNV microarray. We scored 134 high-confidence CNVs across the parasite exome, including 33 deletions and 102 amplifications, which ranged in size from <500bp to 59kb, as well as 10,107 flanking, biallelic SNPs. Overall, CNVs were rare, small and skewed towards low frequency variants, consistent with the deleterious model. Relative to African and SE Asian populations, CNVs were significantly more common in S. America, showed significantly less skew in allele frequencies, and were significantly larger. On this background of low frequency CNV, we also identified several high-frequency CNVs under putative positive selection using an FST outlier analysis. These included known adaptive CNVs containing rh2b and pfmdr1, and several other CNVs (e.g. DNA helicase, and 3 conserved proteins) that require further investigation. Our data are consistent with a significant impact of genetic structure on CNV burden in an important human pathogen. SNP/CGH hybridisation of 175 malaria parasite samples

2015-11-19 | E-GEOD-75137 | biostudies-arrayexpress

Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice

Project description:Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Keywords: knockout effect

2008-03-06 | GSE10740 | GEO

Population structure shapes copy number variation in malaria parasites

2015-11-19 | GSE75137 | GEO

Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals

2008-04-06 | E-GEOD-10740 | biostudies-arrayexpress