Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EXP. 229_AC_RNAseq data of human cancer cell lines 94T778, SKBR3, AU565, SKMEL28 and A375 exposed to the selective MTOR inhibitor torkinib (PP242, Selleckchem, TX, USA) for 72 hours.

ABSTRACT: Drug concentrations were selected to halt cell cycle progression without affecting cell viability: 94T778 10 µM, A375, SKMEL28 and AU565 4 µM, SKBR3 0.8 µM. The pharmacologic inhibition of MTOR repressed expression of the homologous recombination (HR) and Fanconi Anemia (FA) pathways, and selectively high-fidelity but not error-prone DNA polymerases in all cancer cell lines sequenced

ORGANISM(S): Homo sapiens

PROVIDER: GSE148344 | GEO | 2020/04/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

EXP. 229_AC_RNAseq data of human cancer cell lines 94T778, SKBR3, AU565, SKMEL28 and A375 exposed to the selective MTOR inhibitor torkinib (PP242, Selleckchem, TX, USA) for 72 hours.

Project description:EXP. 229_AC_RNAseq data of human cancer cell lines 94T778, SKBR3, AU565, SKMEL28 and A375 exposed to the selective MTOR inhibitor torkinib (PP242, Selleckchem, TX, USA) for 72 hours.

| PRJNA623939 | ENA

Expression data from SKBR3 cells

Project description:Long noncoding RNA plays a significant role in gene regulation. Long noncoding RNA TMS1AS negatively regulates the sense strand gene TMS1 in breast cancer cells SKBR3. TMS1 is invovled in cell growth and apoptosis through regulating its target genes. We used microarrays to detail the global programme of gene expression upon TMS1AS knockdown or TMS1 knockdown and analyzed the specifity of TMS1AS regulating TMS1.

2017-08-01 | GSE85032 | GEO

expression data from NDRG1 depleted SKBR3 cells

Project description:SKBR3 cells expressing NDRG1 shRNA1 or vector control were harvested by trypsinization and total RNA was extracted. Silencing NDRG1 reduces cell proliferation rates, causing lipid metabolism dysfunction including increased fatty acid incorporation into neutral lipids and lipid droplets. global changes in transcriptome due to NDRG1 silencing were observed

2018-06-21 | GSE112841 | GEO

Chromatin immunoprecipitation of human SKBR3 cells using anti-p53 antibodies

Project description:ChIP with anti-p53 (DO-1) of human SKBR3 cells

2010-02-18 | E-MEXP-2538 | biostudies-arrayexpress

RNA-sequencing of SKBR3 cells with and without NTN4 overexpression

Project description:Low NTN4 expression is correlated with breast cancer proliferation and metastasis, while the molecular mechanism of NTN4 has not been fully explored. Here, the breast cancer cells SKBR3 stably expressing dCas9-VP64 and MS-P65-HSG1 were generated by transducing a lentiviral plasmid containing lenti-dCAS-VP64-Blast (Addgene plasmid # 61425) and lenti-EF1a-MS2–p65–HSF1-2A-Hygro-WPRE (Addgene plasmid # 89308), followed by selection and maintained using 10 μg/mL of blasticidin and 200 μg/mL of hygromycin. SKBR3-dCas9-VP64-MPH cells were further transduced with a doxycycline (Dox)-induced expressing sgRNA targeting NTN4 promoter. The successfully transfected cells were selected and maintained with puromycin at a concentration of 0.5 μg/mL for SKBR3-dCas9-VP64-MPH. SKBR3-dCas9-VP64-MPH-AC-sgRNA cells were treated with or without 50 ng/μL Dox (three biological repeats for both groups) to induce NTN4 overexpressing. Total RNA was extracted using TRIzol according to the manufacturer’s instructions. The quality of RNA samples was examined by Agilent BioAnalyser 2100. The samples were subjected to RNA-sequencing analysis on the BGISEQ-500 system by Beijing Genomics Institute (BGI, China). The clean-tag reads were aligned to the reference genome and reference genes using HISAT2 and Bowtie2. The matched reads were calculated and then normalized using RSEM software. Differential gene expression was analyzed using DESeq2 and was carried out further to explore the mechanism of NTN4 in breast cancer.

2022-05-27 | GSE197635 | GEO

TSA induced gene expression variation on breast cancer cell SKBR3

Project description:To explore TSA influence on human breast cancer cells, we attempt to analyze genes differentially expressed between TSA treated and untreated SKBR3 cells, which will hopefully provide clues for TSA target genes.

2017-02-28 | GSE95087 | GEO

Effect of CTTN on SKBR3 breast cancer cells (RNA-seq)

Project description:RNA-sequencing analysis of control and CTTN-overexpressed SKBR3 cell lines. CTTN (also known as EMS1), an actin-binding protein, is highly amplified in several human cancers, including breast carcinomas. Results provide insight into the transcriptional regulation of CTTN in breast cancer.

2023-03-09 | GSE201563 | GEO

expression data from NDRG1 depleted SKBR3 cells

Project description:expression data from NDRG1 depleted SKBR3 cells

| PRJNA449286 | ENA

Proteomic of SKBr3 treated with PFOA or TPP

Project description:Proteomic of SKBr3 cells of Homo sapiens treated by PFOA

2020-05-26 | PXD019397 | iProX

SKBR3 cells treated with epirubicin or hypoxia

Project description:Intratumoral heterogeneity may generate a diversity of resistance mechanisms that could cause different therapeutic responses in different cell populations. Gene expression profiles were generated for breast cancer cells that had survived two different treatment and were also sorted by the cancer stem cell marker CD44. SKBR3 cells were treated with epirubicin or hypoxia, and then flow sorted by CD44 expression and processed for expression profiles. 3 or 4 biological replicates were generated for each condition.

2015-09-09 | E-GEOD-72362 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data