10x 3’ scRNA-seq analysis on Jurkat cells post TCR acivation in CRISPR/Cas9 screening

ABSTRACT: By performing 10x 3’ scRNA-seq on Jurkat cells post TCR acivation in CRISPR/Cas9 screening, we want to investigate the compatibility of the A/G mixed capture sequence on multiple single cell RNA-seq platforms. By mimicking the adenylated endogenous mRNA, gRNA transcripts could be directly captured by poly(dT) primer during the reverse transcription, and serve as perturbation index in high identification rate.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148820 | GEO | 2020/05/11

REPOSITORIES: GEO