RNA sequencing of miRNA in plasma from myocardial ischemia and reperfusion mice and sham mice

ABSTRACT: Purpose: The aim of this study is to compare the plasma miRNA profile between mice subject to myocardial ischemia and reperfusion and mice subject to sham operation. Methods: 8 to 10-week old C57BL/6 mice underwent myocardial ischemia and reperfusion (MIR) or Sham operation. Plasma RNA was isolated using Trizol LS reagent 4h post-surgery. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform.

ORGANISM(S): Mus musculus

PROVIDER: GSE148864 | GEO | 2020/04/18

REPOSITORIES: GEO