Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Efficient Propagation of Circulating Tumor Cells: A First Step for Probing Tumor Metastasis.

ABSTRACT: Circulating Tumor Cells (CTCs) from metastatic breast cancer patients were harvested and grown in vitro for 30+ days in a novel short-term culture method. Additionally, RNA from the buffy coat of 5 healthy donors were sequenced and subjected to culture conditions; 0/5 cultures successfully grew. This new culture method is highly efficient and allows for the molecular characterization of CTCs and identification of potential targets for therapy against tumor metastasis. Using this method, we established 12/12 cultures of CTCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148991 | GEO | 2020/04/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Efficient Propagation of Circulating Tumor Cells: A First Step for Probing Tumor Metastasis.

Project description: Not available

| S-EPMC7599955 | biostudies-literature

Efficient Propagation of Circulating Tumor Cells: A First Step for Probing Tumor Metastasis.

Project description:Efficient Propagation of Circulating Tumor Cells: A First Step for Probing Tumor Metastasis.

| PRJNA626675 | ENA

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 3

Project description:We developed a method to isolate pure circulating tumor cells (CTC). RNA from such CTCs isolated from the peripheral blood of metastatic breats cnacer patients and gene expression was performed using cDNAmicroarray. we used cDNA array to compare gene expression of CTCs with normal epithelial and breast tumor samples CTCs vs. breast tumors

2014-12-18 | E-GEOD-45964 | biostudies-arrayexpress

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 2

2014-12-18 | E-GEOD-45963 | biostudies-arrayexpress

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 1

Project description:We developed a method to isolate pure circulating tumor cells (CTC). RNA from such CTCs isolated from the peripheral blood of metastatic breast cancer patients and gene expression was performed using cDNAmicroarray. we used cDNA array to compare gene expression of CTCs with normal epithelial and breast tumor samples normal blood vs. breast tumor

2014-12-18 | E-GEOD-45962 | biostudies-arrayexpress

Single Cell RNA-sequencing of Pancreatic Circulating Tumor Cells

Project description:Circulating Tumor Cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. Using a pancreatic cancer mouse model, we applied a microfluidic device to isolate CTCs independently of tumor epitopes, subjecting these to single cell RNA-sequencing. This study was conducted to determine the heterogeneity of pancreatic CTCs and to compare these CTCs to matched primary tumors, cell line controls (NB508 cancer cell line and MEF non-cancer cell line), primary tumor single cells, and normal leukocytes/WBCs. We profiled RNA from 75 single cells circulating in mouse blood enriched for circulating tumor cells from 5 mice, 12 single cells from a mouse embryonic fibroblast cell line, 16 single cells from the nb508 mouse pancreatic cancer cell line, 12 single mouse white blood cells, 18 single GFP lineage-traced circulating tumor cells from two mice, 20 single GFP lineage-traced cancer cells from the primary pancreatic tumor of a mouse, and 34 dilutions to 10 or 100 picograms of total RNA from mouse primary pancreatic tumors from 4 mice.

2014-09-22 | E-GEOD-51372 | biostudies-arrayexpress

RNA-Seq of ex vivo culture of circulating breast tumor cells

Project description:Analyses of circulating tumor cells (CTC) cultured from blood of patients with cancer may allow individualized testing for susceptibility to therapeutic regimens. We established ex vivo cultures of CTCs from six patients with metastatic estrogen receptor-positive breast cancer and performed RNA-Seq on those cultures.

2014-07-11 | GSE55807 | GEO

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 3

2014-12-18 | GSE45964 | GEO

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 2

2014-12-18 | GSE45963 | GEO

Isolation and expression profiling of circulating tumor cells in breast cancer patients part 1

2014-12-18 | GSE45962 | GEO