Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism

ABSTRACT: Ribonuclease P (RNase P) is the only ribonuclease responsible for 5'-end maturation of tRNAs in all kingdoms of life. In Escherichia coli, it is also essential for separation of pre-tRNAs from multiple polycistronic transcripts. Using RNA sequencing (RNA-seq), we show here that RNase P affects the abundance of ~44% of the expressed mRNAs demonstrating a much more widespread role in mRNA decay than previously thought. Furthermore, our data also demonstrate for the first time that the polyadenylation of transcripts by PAP I modulates RNase P-mediated mRNA decay as well as tRNA processing.

ORGANISM(S): Escherichia coli

PROVIDER: GSE149024 | GEO | 2021/09/09

REPOSITORIES: GEO