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LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues [hEMB]

ABSTRACT: Using single-cell genomics, we demonstrated that endogenous LINE-1s are mobilized in human pre-implantation embryos

ORGANISM(S): Homo sapiens

PROVIDER: GSE149472 | GEO | 2023/03/31

REPOSITORIES: GEO

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LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-31 | GSE149477 | GEO

LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues [twins]

Project description:We did not detected de novo endogenous LINE-1 insertions in human twins

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LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues [trios]

Project description:We did not detected de novo endogenous LINE-1 insertions in human offsprings

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LINE-1 retrotransposition impacts the genome of human pre-implantation embryos and extraembryonic tissues [placenta]

Project description:We detected de novo endogenous LINE-1 insertions in extraembryonic tissues (chorion frondosum)

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Effect of ZIKV on pre-implantation embryos

Project description:To determine the effect of Zika virus infection on pre-implantation embryonic development, we performed single blastocyst RNA-Seq on MOCK and ZIKV infected embryos. ZIKV infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the mechanisms underlying fetal loss remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pregnancy, or pre- and peri-implantation, because most current studies of ZIKV infection in pregnancy models focus on post-implantation stages. Here, we demonstrate that trophectoderm cells of pre-implantation human and mouse embryos can be efficiently infected with ZIKV, and that trophectoderm can propagate virus causing cell death of neural progenitors. These findings were corroborated by our demonstration that hESC-derived trophectoderm cells are infected by ZIKV in a dose dependent manner. RNAseq of single blastocysts revealed key transcriptional changes in cellular and physiologic functions upon ZIKV infection, including nervous system development and function, prior to commitment to the neural cell lineage. Finally, the pregnancy rate of mice infected pre-implantation was > 50% lower than females infected at E4.5. These results demonstrate that pre-implantation ZIKV infection of trophectoderm leads to miscarriage or spontaneous abortion. Moreover, pre- and peri-implantation ZIKV infects trophectoderm cells that propagate virus over time causing cell death in neural progenitors. Cumulatively, these data demonstrate that vertical pre- and peri-implantation ZIKV infection of trophectoderm impairs fetal development and causes neural progenitor cell death, elucidating a previously unappreciated association of pre- and peri-implantation ZIKV infection and microcephaly.

2019-08-16 | GSE133254 | GEO

Effect of ZIKV on pre-implantation embryos

Project description:Effect of ZIKV on pre-implantation embryos

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Expression data from Human Pre-implantation embryos

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Isolation of epiblast stem cells from pre-implantation mouse embryos

Project description:Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are different than mouse ES cells and share defining features with EpiSCs, yet are derived from pre-implantation human embryos. Here we show that EpiSCs can be routinely derived from pre-implantation mouse embryos. The pre-implantation-derived EpiSCs exhibit molecular features and functional properties consistent with bona fide EpiSCs. These results provide a simple method for isolating EpiSCs and offer direct insight into the intrinsic and extrinsic mechanisms that regulate the acquisition of distinct pluripotent states.

2011-01-25 | GSE26814 | GEO

Isolation of epiblast stem cells from pre-implantation mouse embryos

2011-01-25 | E-GEOD-26814 | biostudies-arrayexpress

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