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Small RNA sequencing of plasma miRNA from sepsis patients

ABSTRACT: Purpose: The aim of this study is to compare the plasma miRNA profile between healthy control and sepsis patients Methods: Plasma from healthy control and sepsis patients were used in the study. Total RNA was isolated from equal volume of plasma using Trizol LS. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform.

ORGANISM(S): Homo sapiens

PROVIDER: GSE149764 | GEO | 2020/05/04

REPOSITORIES: GEO

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