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RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

ABSTRACT: Purpose: To uncover immune mediated genes and pathways by Pseudomonas aeruginosa infection that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C. elegans Methods: RNA was extracted from synchronized Pseudomonas aeruginosa infected L4 stage unc-30(ok613) and WT using Qiagen extraction kits and following standard methods. The animals were grown on OP50 at 20 C and infected at 25 C. Results: RNA seq analyses shows enriched and signficant Pseudomonas aeruginosa mediated upregulated immune, neuropeptide and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans during Pseudomonas aeruginosa infection

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE149862 | GEO | 2021/01/31

REPOSITORIES: GEO

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RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C elegans

Project description:Purpose: To uncover immune and longevity genes and pathways that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C elegans Methods: RNA was extracted from synchronized L4 stage unc-30(ok613) and WT animals grown at 20 C using Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, ageing and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans

2021-05-05 | GSE149742 | GEO

RNA sequencing to identify UNC-25-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

Project description:Purpose: To gain molecular insights on how UNC-25 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-25(e156) animals relative to wild-type animals with or without P. aeruginosa (PA14) infection. Methods: Synchronized L1 worms were placed onto NGM plates seeded with E. coli OP50 and grown at 20 °C until the L4 stage. Worms were washed off the plates, collected with M9 solution and subsequently transferred to modified NGM plates containing E. coli OP50 or P. aeruginosa PA14 for 24 h at 25 °C. Then, the animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01, China) with liquid nitrogen. Total RNA was isolated using a TransZol Up Plus RNA kit (Transgen, ER501-01, China). Results: RNA seq analyses shows enriched and signficant changed immune genes, neuropeptides, transcription factors and pathways that are dependent of GABA signaling. Conclusion: Our studies uncover that GABA signaling enhance the resistence to pathogen induced killing.

2023-06-26 | GSE195835 | GEO

RNA sequencing to identify UNC-49-regulated genes in C. elegans defense against pathogen infection

Project description:To gain molecular insights on how UNC-49 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-49(e407) animals relative to wild-type animals with or without P. aeruginosa(PA14) infection. We found that UNC-49 suppresses the expression of insulin pathway genes, and lack of UNC-49-mediated suppression in unc-49(e407) animals contributes to their improved survival against P. aeruginosa infection.

2023-10-23 | GSE146406 | GEO

ChIP-seq of UNC-30 and UNC-55 in C.elegans

Project description:We performed ChIP-seq for GFP fusion protein of UNC-30 and UNC-55 with endogenous expression

2017-08-28 | GSE102213 | GEO

ChIP-Seq of UNC-3 in C. elegans

Project description:We performed ChIP-seq on endogenously tagged UNC-3::GFP fusion proteins in C. elegans

2020-10-01 | GSE143165 | GEO

RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

Project description:RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C. elegans in Pseudomonas aeruginosa (PA14) infection

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RNA sequencing of cell-specific profile of wild type and unc-4 VAs

Project description:We report the transcriptome profile of the VA neuron in L2 wild type and unc-4 mutant C. elegans

2021-09-01 | GSE173287 | GEO

RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C elegans

Project description:RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C elegans

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Network modeling reveals genetic interactions that influence C. elegans defense against infection

Project description:Time respoved RNA-seq during Caenorhabditis elegans infection by Pseudomonas aeruginosa PA14.

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ChIP-Seq of UNC-3 in C. elegans

Project description:ChIP-Seq of UNC-3 in C. elegans

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