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Identification of SHOT1/MTERF18 binding sites on the mitochondrial genome of Arabidopsis thaliana

ABSTRACT: To identify possible mitochondrial DNA binding targets of SHOT1/MTERF18 (AT3G60400), C-terminal GFP fusion constructs under either a constitutive 35S promoter (35S::SHOT1-GFP) or the SHOT1 native promoter (SHOT1p::SHOT1-GFP) were made. The transgenes were introduced into the shot1-2 mutant background (Kim et al., 2012, Plant Cell) and verified to be functional based on recovery of shot1 from growth defects. As a control, a transgenic line harboring mitochondria-targeted GFP (Mito-GFP) under the 35S promoter was used. All enriched peaks in SHOT1-GFP samples are located upstream of different tRNA genes: trnS, trnM, trnG and trnF

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE150262 | GEO | 2020/08/09

REPOSITORIES: GEO

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