Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A404 Smooth Muscle Cell Differentiation

ABSTRACT: In vitro time course experiment using the A404 clone of P19 mouse embryonal carcinoma cells to evaluate SMC differentiation in response to retinoic acid followed by phenotype-specific selection with puromycin. Four time points of 5-6 biological replicates each were performed. Keywords: time-course

ORGANISM(S): Mus musculus

PROVIDER: GSE1506 | GEO | 2004/06/30

SECONDARY ACCESSION(S): PRJNA90833

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

P19-atRA-kinetics

Project description:P19 embryonal carcinoma cells were treated with 1µM all trans retinoic acid and total RNA was collected at different time points

2013-06-30 | E-MEXP-3669 | biostudies-arrayexpress

Physiological and morphological status of cells dictates RNA biosignature of their exosomes

Project description:P19 cells, a pluripotent embryonal cell line derived from mouse teratocarcinoma, can be stimulated with retinoic acid to induce neural differentiation forming P19 neurons (McBurney et al., 1982). P19 cells (undifferentiated cells abbreviated as UD-P19 cells) and P19 neurons were cultured in a defined medium lacking fetal bovine serum and conditioned medium was collected. Exosomes were purified from conditioned medium by ultracentrifugation method. Exosomes were characterized using a number of techniques according to the guidelines of ISEV. RNA was purified from exosomes using miRCURY™ RNA isolation kit (Exiqon, Inc.; now Qiagen) according to the instructions of the Supplier. Exosomal RNA concentration was measured using NanoDrop™ 8000 spectrophotometer. The approximate size of exosomal RNAs was analyzed on BioAnalyzer and by 5' end labeling and their separation on denaturing gel. Differential expression of RNAs in exosomes from P19 cells and P19 neurons was determined by RNA-Seq method. Double stranded barcoded cDNA libraries were generated and sequenced on Illumina NextSeq 500 platform. Three biological replicates were used from P19 cells. P19 neurons exosomes were from P19 neurons at 8, 10, 12 days of differentiation. RNA-Seq data was analyzed using bioinformatics pipeline.

2023-02-27 | GSE152655 | GEO

Neuroblastoma Neuro2A Cell Differentiation induced by retinoic acid-treatment

Project description:To investigate a role of mKIAA genes in early stage of neurite outgrowth, in vitro time course experiment was performed using the mouse neuroblastoma Neuro2A cells and all-trans-retinoic acid. Six time points of 8 biological replicates were performed.These data were normalized by median intensities and subtracted background intensities. NOTE: Outlier involved several data were not deposited. Keywords: time-course

2005-04-28 | GSE2570 | GEO

P19 embryonic carcinoma (EC) cells induced to form cardiomyocytes in vitro

Project description:SAGE identification of differentiation responsive genes in P19 embryonic cells induced to form cardiomyocytes in vitro. P19 embryonic carcinoma (EC) cells, induced to form cardiomyocytes in vitro - undifferentiated cells, day 3+0.5 and day 3+3.0 of differentiation protocol. Keywords = EC cells, P19, differentiation, cardiomyocytes Keywords: time-course

2002-07-18 | GSE42 | GEO

Effect of LIN28 on miRNA profile at day 5 of neurogliogenesis in vitro

Project description:Comparison of mouse P19 and P19+Lin28 cells at day 5 of retinoic acid-induced differentiation The cell lines differ in that one constitutively expresses Lin28 from a heterologous promoter.

2010-02-25 | GSE19858 | GEO

P19 embryonic carcinoma (EC) cells induced to form cardiomyocytes in vitro

2002-07-18 | E-GEOD-42 | biostudies-arrayexpress

Whole-genome gene expression level changes in P19.6 mouse embryonal carcinoma cells compared to P19.6 cells treated for 48 hours with all-trans retinoic acid

Project description:Enhancers are developmentally-controlled transcriptional regulatory regions whose activities are modulated through histone modifications or histone variant deposition. Here, we show by genome-wide mapping that the newly discovered DNA modification 5-hydroxymethylcytosine (5hmC) is dynamically associated with transcription factor binding to distal regulatory sites during neural differentiation of mouse P19 cells as well as during adipocyte differentiation of mouse 3T3-L1 cells. Functional annotation reveals that regions gaining 5hmC are associated with genes expressed either in neural tissues when P19 cells undergo neural differentiation or in adipose tissue when 3T3-L1 cells undergo adipocyte differentiation. Furthermore, distal regions gaining 5hmC together with H3K4me2 and H3K27ac in P19 cells behave as differentiation-dependent transcriptional enhancers. Identified regions are enriched in motifs for transcription factors regulating specific cell fates like Meis1 in P19 cells and PPARgamma in 3T3-L1 cells. Accordingly, a fraction of hydroxymethylated Meis1 sites were associated with a dynamic engagement of the 5mC hydroxylase Tet1. In addition, kinetic studies of cytosine hydroxymethylation of selected enhancers indicated that DNA hydroxymethylation is an early event of enhancer activation. Hence, acquisition of 5hmC in cell-specific distal regulatory regions may represent a major event of enhancer progression toward an active state and participate in selective activation of tissue-specific genes A 6-chip study aiming to characterize regulated genes in P19.6 mouse embryonal carcinoma cells following 48 hours treatment with 1M-BM-5M all-trans retinoic acid. RNAs were prepared from three independent triplicate experiments.

2012-06-03 | E-GEOD-27334 | biostudies-arrayexpress

Time course RA-treatment of B16 mouse melanoma cells

Project description:Time course experiment for treatment of B16 mouse melanoma cells with all-trans retinoic acid Keywords: Time couse Time-course experiment with treatment at 4hr, 10hr, 24hr, and 48hr. Six biological replicates per time point; one biological replicate per array. Dye swap.

2008-07-31 | E-GEOD-11580 | biostudies-arrayexpress

Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

Project description:To gain a deeper insight into dynamic changes of H3K9cr and H3K9ac during cell fate commitment, ChIP-seq was performed to analyze the genome-wide changes of H3K9cr and H3K9ac during neuroectodermal differentiation of the P19 embryonal carcinoma cells.

2022-04-17 | GSE200519 | GEO

RIME against PBX1 protein in P19.6 mouse embryonal carcinoma cells treated for 48 hours with all-trans retinoic acid

Project description:Identification of PBX1 interactors in P19.6 mouse embryonal carcinoma cells treated for 48 hours with all-trans retinoic acid.

2017-04-03 | PXD006158 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data