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Bac2 mutant transcriptome modification during osmotic stress

ABSTRACT: rs08-05_bac2 - bac2-catma - We are studying the function of a gene encoding a mitochondrial basic amino acid carrier , BAC2 in Arabdopsis thaliana.This gene is expressed during osmotic and salt stresses, and dark-induced senescence. We wish to determine whether there are transcriptome modifications during osmotic stress in mutant plants (bac2) compared to wild-type plants . - Mutant and Wild-type plants were grown on grids for 7 days on plates containing MS/2 and saccharose. Plants were transferred for 24h to liquid medium containing MS/2 saccharose (untreated plants) or 0.4M mannitol MS/2 saccharose (treated plants). Keywords: gene knock out,treated vs untreated comparison,wt vs mutant comparison

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE15063 | GEO | 2009/10/01

SECONDARY ACCESSION(S): PRJNA114835

REPOSITORIES: GEO

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bac2 mutant transcriptome modification during osmotic stress

Project description:rs08-05_bac2 - bac2-catma - We are studying the function of a gene encoding a mitochondrial basic amino acid carrier , BAC2 in Arabdopsis thaliana.This gene is expressed during osmotic and salt stresses, and dark-induced senescence. We wish to determine whether there are transcriptome modifications during osmotic stress in mutant plants (bac2) compared to wild-type plants . - Mutant and Wild-type plants were grown on grids for 7 days on plates containing MS/2 and saccharose. Plants were transferred for 24h to liquid medium containing MS/2 saccharose (untreated plants) or 0.4M mannitol MS/2 saccharose (treated plants). Keywords: gene knock out,treated vs untreated comparison,wt vs mutant comparison 8 dye-swap - CATMA arrays

2009-10-01 | E-GEOD-15063 | biostudies-arrayexpress

Adaptation of Root Architecture to Abiotic Stress

Project description:rs08-01_met2 - comparative analysis of catma chips with a met2 overexpression line - Role of the MET2 methyltransferase on methylation and gene expression in arabidopsis - Comparison of transcription in roots and aerial parts of one week-old plants from two lines (WT and MET2 overexpressor) Keywords: organ comparison,wt vs mutant comparison

2009-05-28 | GSE11563 | GEO

ralstonia infection-characterisation of wat1 mutant

Project description:rs08-04_wat1-ralstonia - ralstonia infection - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - Comparison between the mutant and the wt at different time after infection with ralstonia bacteria Keywords: treated vs untreated comparison 20 dye-swap - CATMA arrays

2010-07-31 | E-GEOD-19081 | biostudies-arrayexpress

mutant comparison - characterisation of wat1 mutant

Project description:rs08-04_wat1-ralstonia - mutant comparison - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - wt versus mutant comparison in the leaf and the root Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays

2010-07-01 | E-GEOD-19077 | biostudies-arrayexpress

ralstonia infection-characterisation of wat1 mutant

2010-08-01 | GSE19081 | GEO

Adaptation of Root Architecture to Abiotic Stress

2010-05-18 | E-GEOD-11563 | biostudies-arrayexpress

mutant comparison - characterisation of wat1 mutant

2010-07-01 | GSE19077 | GEO

Catma1-bou-bou mutant characterisation: Autotrophic growth acquisition in the ABOUTDESOUFFLE (bou) mutant

Project description:rs07-09_bou - catma1-bou - Autotrophic growth acquisition is abolished in the bou mutant in Arabidopsis thaliana. BOU encodes a putative mitochondrial acyl carnitine carrier. bou mutant is blocked at the cotyledon stage. Autotrophic growth of the bou mutant can be achieved with addition of sugar in the medium or in darkness. Moreover, BOU gene expression is activated by light and depends on plant developmental stage. We wish to determine what are the consequences of bou gene mutation at the transcriptome level. We wish to understand whether bou growth arrest is due to the modification of specific genes expression or to a general effect on metabolism at the transition from heterotrophic to autotrophic growth. - Seeds from a heterozygous plants were grown for either 5 or 8 days after germination on synthetic medium (MS/2) without sugar under continuous light. We harvested cotyledon-stage blocked plants (bou phenotype) from three independent Petri dishes and also green seedlings with true leaves and fully developed root (heterozygotes with a wild-type phenotype) . We also grew independently Col-O plants for 5 and 8 days to compare them with the bou mutants. Keywords: gene knock in (transgenic),normal vs disease comparison,time course 5 dye-swap - CATMA arrays

2009-02-05 | E-GEOD-10399 | biostudies-arrayexpress

Response to salt stress of Raptor mutants

Project description:ra12-03_raptor-nacl - Characterization to raptor mutants (partner of the kinase TOR) and of their answer to the salt stress. - Sowing on metal grids on MS/2 medium with continuous light. Transfer after 12 days of culture for 3h to liquid MS/2 medium containing or not 200 mM NaCl. 7 dye-swap - gene knock out, treated vs untreated comparison

2013-07-01 | E-GEOD-47391 | biostudies-arrayexpress

Catma1-bou-bou mutant characterisation: Autotrophic growth acquisition in the ABOUTDESOUFFLE (bou) mutant

2009-02-05 | GSE10399 | GEO

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