Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression changes of Planococcus kocurii O516 in various culture conditions (with or without sulfamethoxazole)

ABSTRACT: The strain Planococcus kocurii O516 isolated from the marine culture environment was found to be stably and highly effective for the degradation of sulfamethoxazole. We investigated the expression behavior of functional genes related to this degradation ability in P. kocurii O516 under the culture conditions with or without sulfamethoxazole.

ORGANISM(S): Planococcus kocurii

PROVIDER: GSE150668 | GEO | 2020/08/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression changes of Planococcus kocurii O516 in various culture conditions (with or without sulfamethoxazole)

Project description:Gene expression changes of Planococcus kocurii O516 in various culture conditions (with or without sulfamethoxazole)

| PRJNA633128 | ENA

Transcriptional Response of Mycobacterium tuberculosis H37Rv to sulfamethoxazole

Project description:To understand the response of M. tuberculosis (MTB) to the drug sulfamethoxazole, we performed transcriptomics on MTB bacilli exposed to the drug.

2018-08-04 | GSE118100 | GEO

Gene expression profile of Vibrio cholerae N16961 grown in various conditions of culture

Project description:To investigate the effect of conditions of culture on gene expression in Vibrio cholerae N16961 We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 conditions of culture in biological duplicate

2023-02-20 | GSE214813 | GEO

Sulfamethoxazole triggers specific microbial activities under aerobic heterotrophic conditions: A metaproteomic approach

Project description:The global sanitary crisis derived from antibiotic multi-resistant bacteria entails the need to reduce sulfamethoxazole (SMX) concentrations in wastewater treatment plants (WWTPs). The key microorganisms and the biotransformation mechanisms leading to SMX removal remain incompletely characterized, particularly under aerobic heterotrophic conditions, which are becoming increasingly relevant in the design of novel, more energy-efficient, WWTPs. In this study, sequential batch reactors were inoculated with activated sludge, operated in heterotrophic conditions and spiked with six different initial SMX concentrations ranging between 0 and 2000 µg L-1. The goal was to determine the influence of SMX in the microbiome and its enzymatic expression through genomic, metaproteomic and transformation product analyses. The results allowed us to identify the metabolite 2,4(1H,3H)-pteridinedione-SMX (PtO-SMX), pointing to the role of the pterin-conjugation pathway in the biotransformation of SMX. Additionally, at increased SMX concentrations, through metaproteomics and 16S rRNA gene sequencing, it was determined a higher abundance of the genus Corynebacterium and a differential expression of five enzymes involved in its central metabolism, suggesting the relevant role of this bacteria to mitigate SMX risks.

2022-09-20 | PXD029711 | Pride

Effect of culture/microexpansion in various conditions on virus-specific CD8+ T cells

Project description:To investigate the conditions for the optimal expansion ot T cell therapeutic products, we used a microexpansion platform to screen various cytokine cocktails (no cytokines, IL-4 + IL-7, IL-15, IL-15 + IL-4, IL-15 + IL-6, and IL-15 + IL-7). We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different donors under 6 different culture/microexpansion conditions

2023-01-19 | GSE223140 | GEO

Effect of culture/microexpansion in various conditions on virus-specific CD4+ T cells

2023-01-19 | GSE223139 | GEO

Gene expression changes of Pseudomonas syringae pv. actinidiae biovar 6 in various culture conditions

Project description:Pseudomonas syringae pv. actinidiae biovar 6 (Psa6) is a causal agent of kiwifruit bacterial canker and is a unique plant pathogenic bacterium, producing two types of phytotoxins, coronatine and phaseolotoxin. We investigated the expression behavior of virulent genes of Psa6 under various culture conditions.

2020-05-02 | GSE149743 | GEO

Multiomics study to elucidate the biodegradation of copolyester film by a marine microbial enrichment culture

Project description:Although the biodegradation of biodegradable plastics in soil and compost is well-studied, there is little knowledge on the metabolic mechanisms of synthetic polymers degradation by marine microorganisms. Here, we present a multiomics study to elucidate the biodegradation mechanism of a commercial aromatic-aliphatic copolyester film by a marine microbial enrichment culture. The plastic film and each monomer can be used as sole carbon source. Our analysis showed that the consortium synergistically degrades the polymer, different degradation steps being performed by different members of the community. Analysis of gene expression and translation profiles revealed that the relevant degradation processes in the marine consortium are closely related to poly(ethylene terephthalate) biodegradation from terrestrial microbes. Although there are multiple genes and organisms with the potential to perform a degradation step, only a few of these are active during biodegradation. Our results elucidate the potential of marine microorganisms to mineralize biodegradable plastic polymers and describe the mechanisms of labor division within the community to get maximum energetic yield from a complex synthetic substrate.

2020-09-25 | PXD018391 | Pride

Establishment of trophoblast stem cells under defined culture conditions in mice

Project description:The inner cell mass (ICM) and trophoblast cell lineages duet early embryonic development in mammals. After implantation, the ICM forms the embryo proper as well as some extraembryonic tissues, whereas the trophoectoderm (TE) exclusively forms the fetal portion of the placenta and the trophoblast giant cells. Although embryonic stem (ES) cells can be derived from ICM in cultures of mouse blastocysts in the presence of LIF and/or combinations of small-molecule chemical compounds, and the undifferentiated pluripotent state can be stably maintained without use of serum and feeder cells, defined culture conditions for derivation and maintenance of undifferentiated trophoblast stem (TS) cells have not been established. Here, we report that addition of FGF2, activin A, XAV939, and Y27632 are necessary and sufficient for derivation of TS cells from both of E3.5 blastocysts and E6.5 early postimplantation extraembryonic ectoderm. Moreover, the undifferentiated TS cell state can be stably maintained in chemically defined culture conditions. Cells derived in this manner expressed TS cell marker genes, including Eomes, Elf5, Cdx2, Klf5, Cdh1, Esrrb, Sox2, and Tcfap2c; differentiated into all trophoblast subtypes (trophoblast giant cells, spongiotrophoblast, and labyrinthine trophoblast) in vitro; and exclusively contributed to trophoblast lineages in chimeric animals. This delineation of minimal requirements for derivation and self-renewal provides a defined platform for precise description and dissection of the molecular state of TS cells.

2014-07-08 | GSE59107 | GEO

Transcriptional Response of Mycobacterium tuberculosis H37Rv to sulfamethoxazole

Project description:Transcriptional Response of Mycobacterium tuberculosis H37Rv to sulfamethoxazole

| PRJNA484405 | ENA