RNA-seq for rapid response to glyphosate in Ambrosia trifida

ABSTRACT: Giant ragweed (Ambrosia trifida) is an economically disruptive weed in US corn and soybean production. Populations of this species have evolved a unique glyphosate resistance mechanism involving rapid cell death in mature leaves, also known as rapid necrosis. This abiotic stress resistance mechanism may be due to utilization of stress response pathways typically associated with biotic stresses such as pathogens. To study the genetic basis of this rapid response, an RNA-Seq time course study was conducted on young and mature leaves of glyphosate-resistant and -susceptible individuals, before and after glyphosate treatment. A major difference in gene regulation following glyphosate application was observed between young and mature leaves in the glyphosate-resistant individuals. Many differentially expressed genes were related to broad plant pathways with cross-functionality including plant stress and cell death pathways, phytohormone synthesis, response to oxidative stress, cellular metabolism, and pathogen defense pathways. Metabolic network analysis revealed that most of the genes involved in jasmonate biosynthesis were up regulated in response to glyphosate treatment in resistant mature leaves whereas this pathway was not induced in susceptible plants. The glyphosate-resistant plants appear to perceive glyphosate differently than susceptible plants, pointing to the potential role of a yet to be characterized receptor that senses glyphosate or its action and triggers the rapid necrosis response in mature leaves. Additional steps are needed for functional validation to test the hypothesis of causal relationship between function of these candidate genes of interest and the glyphosate resistance rapid response phenotype.

ORGANISM(S): Ambrosia trifida

PROVIDER: GSE150914 | GEO | 2025/12/31

REPOSITORIES: GEO