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Cell cycle and cell death interaction in plants: PCNA, AtXRs and AtMIPS pathway.

ABSTRACT: rs07-04_mips - atmips mutant/photoperiod - In Arabidopsis thaliana, how does MIPS knock out influence gene expression? In AtMIPS mutants how does photoperiod influence gene expression. - Wild type Colombia and MIPS mutant (Salk 023626) plants are grown in short days (8h light) during 27 days and then transfered in long days (16h light) during 4 days. J4JC correspond to 27+4=31 days plants in short days. Keywords: gene knock out

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE15123 | GEO | 2009/03/07

SECONDARY ACCESSION(S): PRJNA114879

REPOSITORIES: GEO

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Cell cycle and cell death interaction in plants: PCNA, AtXRs and AtMIPS pathway.

Project description:rs07-04_mips - atmips mutant/photoperiod - In Arabidopsis thaliana, how does MIPS knock out influence gene expression? In AtMIPS mutants how does photoperiod influence gene expression. - Wild type Colombia and MIPS mutant (Salk 023626) plants are grown in short days (8h light) during 27 days and then transfered in long days (16h light) during 4 days. J4JC correspond to 27+4=31 days plants in short days. Keywords: gene knock out 6 dye-swap - CATMA arrays

2009-03-07 | E-GEOD-15123 | biostudies-arrayexpress

apc transcriptome-Transcriptome on Arabidopsis APC hypomorphic lines

Project description:rs07-03_apc - apc transcriptome - Which genes are misregulated when the APC complex activity is reduced? - Wild Type Col0, APC10S and CDC16S lines (down regulation of APC10 and CDC16 endogenous genes due to overexpression of a sequence corresponding to a part of APC10 or CDC16 gene, respectively) are grown in growth chamber (16h photoperiod 22°C) for 15 days. Whole seedlings are then frozen in liquid nitrogen and total RNA are extracted.

2012-03-27 | GSE36653 | GEO

Down regulation of qptgene in arabidopsis-Regulation of NAD biosynthesis in Arabidopsis : role of quinolinate phosphoribosyltransferase.

Project description:rs11-08_nad2 - down regulation of qptgene in arabidopsis - Study of the biosynthesis of NAD in Arabidopsis. Involvment of QPTgene using the knock out Salk mutant N575260. - Study of the biosynthesis of NAD in Arabidopsis. Involvment of QPTgene using the knock out Salk mutant N575260.

2013-08-01 | GSE49034 | GEO

apc transcriptome-Transcriptome on Arabidopsis APC hypomorphic lines

Project description:rs07-03_apc - apc transcriptome - Which genes are misregulated when the APC complex activity is reduced? - Wild Type Col0, APC10S and CDC16S lines (down regulation of APC10 and CDC16 endogenous genes due to overexpression of a sequence corresponding to a part of APC10 or CDC16 gene, respectively) are grown in growth chamber (16h photoperiod 22Â°C) for 15 days. Whole seedlings are then frozen in liquid nitrogen and total RNA are extracted. 4 dye-swap - normal vs rnai mutant comparison

2012-03-26 | E-GEOD-36653 | biostudies-arrayexpress

pICln, an spliceosome assembly chaperone, controls alternative splicing, flowering time and stress responses in Arabidopsis

Project description:RNA-Seq from picln-1 mutant, prmt5-5 mutant and wt grown at 22°C for 9 days in a 12h light 12h dark photoperiod

2022-06-30 | GSE130463 | GEO

Down regulation of qptgene in arabidopsis-Regulation of NAD biosynthesis in Arabidopsis : role of quinolinate phosphoribosyltransferase.

2013-08-01 | E-GEOD-49034 | biostudies-arrayexpress

Transcript profiling in Arabidopsis gollum mutant: Role of the Gollum gene in the plant development

Project description:rs07-08_gollum - trancript profiling in arabidopsis gollum mutant. Elucidate the role of Gollum gene in the plant development by a RNAi approach. Seeds of WT and RNAi lines were sowed on soil and conserved 2 days at 4°C. The pots were transferred to the greenhouse under long-day conditions (16h light, 22°C). The leaves of one-month old plants were harvested and total RNA were extracted. Keywords: wt vs mutant comparison

2008-11-01 | GSE13417 | GEO

knock out mutant n575260-NAD biosynthesis in Arabidopsis : role of quinolinate phosphoribosyltransferase

Project description:rs11-01_nad - knock out mutant n575260 - Study of the impact of a deregulation in the NAD concentration in arabidopsis - Study of the biosynthesis of NAD in Arabidopsis. Involvment of QPRTgene using the knock out mutant N575260 from Salk.

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Catma1-bou-bou mutant characterisation: Autotrophic growth acquisition in the ABOUTDESOUFFLE (bou) mutant

Project description:rs07-09_bou - catma1-bou - Autotrophic growth acquisition is abolished in the bou mutant in Arabidopsis thaliana. BOU encodes a putative mitochondrial acyl carnitine carrier. bou mutant is blocked at the cotyledon stage. Autotrophic growth of the bou mutant can be achieved with addition of sugar in the medium or in darkness. Moreover, BOU gene expression is activated by light and depends on plant developmental stage. We wish to determine what are the consequences of bou gene mutation at the transcriptome level. We wish to understand whether bou growth arrest is due to the modification of specific genes expression or to a general effect on metabolism at the transition from heterotrophic to autotrophic growth. - Seeds from a heterozygous plants were grown for either 5 or 8 days after germination on synthetic medium (MS/2) without sugar under continuous light. We harvested cotyledon-stage blocked plants (bou phenotype) from three independent Petri dishes and also green seedlings with true leaves and fully developed root (heterozygotes with a wild-type phenotype) . We also grew independently Col-O plants for 5 and 8 days to compare them with the bou mutants. Keywords: gene knock in (transgenic),normal vs disease comparison,time course

2009-02-05 | GSE10399 | GEO

INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptors HAE/HSL2 are required for cell expansion and cell separation during floral organ abscission in Arabidopsis thaliana (part 2)

Project description:Plants have the ability to shed organs that are no longer in use. In Arabidopsis thaliana abscission of floral organs involves cell wall remodeling and cell expansion prior to cell wall dissolution. IDA encodes a secreted peptide that signals through the leucine-rich repeat receptor-like kinases (LRR-RLKs) HAESA (HAE) (At4g28490) and HASEA-LIKE2 (HSL2) (At5g65710). Arabidopsis thaliana (ecotype Colombia-0) plants were kept in growth chambers with a 16/8 h (light/dark) photoperiod at 22 M-BM-0C, and 100 mE m-2 s-1 light intensity. 4 biological replicates were prepared from each sample, each containing abscission zone regions of siliques position 4 to 8 (when counting from the flowe at anthesis at the top of the inflorescence) from plants with at least 20 siliques. Differences in transcriptional responses were measured by comparing genes expression in abscission zones of ida-2 plants (SALK_133209) against abscission zones from control plants.

2013-12-30 | E-GEOD-48107 | biostudies-arrayexpress

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