Phosphorylation of the transcriptional coregulator alphaNAC by DNA-PKcs regulates osteoblastogenesis
ABSTRACT: In order to identify pathways affected by inhibition of the DNA-dependent protein kinase, catalytic subunit in osteoblasts, we performed a whole genome gene expression profiling using the Affymetrix mouse GeneChip system (MoGene-2.0 gene ST arrays). The osteoblastic cell line MC3T3-E1 was grown in osteogenic medium and treated with DMSO (vehicle) or NU7441, an inhibitor of DNA-dependent protein kinase, for 14 days. Six biological samples were used in this experiment, three replicates in the control group and three replicates in the treated group. Isolated RNA was subjected to microarray analysis. Overall design: In total 6 samples; 3 controls and 3 treatment.