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20

Chip-chip from pro-B cells with H3K4me3, H3K4me2 and H3K9ac


ABSTRACT: The transcription factor Pax5 represses B-lineage-inappropriate genes and activates B-cell-specific genes in B-lymphocytes. Here we have identified 170 novel Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expression in pro-B and mature B cells. Expression of half of the Pax5-activated genes is either absent or significantly reduced upon Pax5 loss in plasma cells. Direct Pax5 target genes were identified based on their protein synthesis-independent activation by a Pax5-estrogen receptor fusion protein. Chromatin immunoprecipitation (ChIP) of Pax5 together with chromatin profiling by ChIP-on-chip analysis demonstrated that Pax5 directly activates the chromatin at promoters or putative enhancers of Pax5 target genes. The novel Pax5-activated genes code for key regulatory and structural proteins involved in B cell signaling, adhesion, migration, antigen presentation and germinal center B cell formation, thus revealing a complex regulatory network, which is activated by Pax5 to control B cell development and function. Keywords: Chip-chip, cell type comparison Overall design: comparison of Pax5-/-Rag2-/- vs Rag2-/- pro-B cells

INSTRUMENT(S): GenAu MB BCELL v2.0

ORGANISM(S): Mus musculus  

SUBMITTER: Meinrad Busslinger   

PROVIDER: GSE15145 | GEO | 2009-04-27

SECONDARY ACCESSION(S): PRJNA114909

REPOSITORIES: GEO

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