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Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach

ABSTRACT: Seasonal weight loss is one of the most important limitations to ruminant production in drought-prone regions. In these regions and under the dry season, animals may lose up to 30% of their body weight, conditioning production performance. It is of the utmost importance to understand the physiological basis of tolerance to SWL, particularly by contrasting breeds that are susceptible and tolerant to SWL. In this study, transcriptomics was used to describe the gene expression effects of feed restriction in dairy goat of two breeds with different SWL tolerance: Majorera (tolerant) and Palmera (susceptible). Two control and two restricted groups per breed were established: Majorera Control (adequately fed; MC; n=4), Palmera Control (adequately fed; PC; n=6), Majorera Restricted (feed restricted; ME; n=5) and Palmera Restricted (feed restricted; PE; n=4). On day 22 of the trial, mammary gland biopsies were collected for transcriptomics analysis. Results were obtained using Next Generation RNA sequencing and validation using qPCR.

ORGANISM(S): Capra hircus

PROVIDER: GSE151599 | GEO | 2020/07/24

REPOSITORIES: GEO

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Project description:Background: Soil salinity is a major abiotic stress factor that limit agricultural productivity worldwide, and this problem is expected to grow in the future. Common bean (Phaseolus vulgaris L.) is an important protein source in developing countries is highly susceptible to salt stress. To understand the underlying mechanism of salt stress responses, transcriptomics, metabolomics, and ion content analysis were utilized for response comparison of salt-tolerant and salt-susceptible common bean genotypes in saline conditions. Results: Transcriptome analysis has revealed that the tolerant genotype had increased photosynthesis in saline conditions while the susceptible genotype acted in a contrasting way. The chlorophyll content measurements have backed up this result with increase in tolerant and decrease in susceptible genotype. Transcriptome also displayed a more active carbon and amino acid metabolism for the tolerant genotype as well. Analysis of primary metabolites with GC-MS demonstrated the boosted carbohydrate metabolism in the tolerant genotype with increased sugar content as well as better amino-acid metabolism with the accumulation of glutamate and asparagine and hinted a lowered photorespiration level for the tolerant one. Accumulation of lysine, valine, and isoleucine in the roots of the susceptible genotype suggested a halted stress response pathway. According to ion content comparison, the tolerant genotype managed to block accumulation of Na+ in the leaves while accumulating significantly less Na+ in the roots compared to susceptible genotype. K+ levels increased in the leaves of both genotype and the roots of the susceptible one but dropped in the roots of the tolerant genotype. Additionally, Zn+2 and Mn+2 levels were also dropped in the tolerant roots, while Mo+2 levels were significantly higher in all tissues in both control and saline conditions for tolerant genotype. Conclusion:The results of the presented study have demonstrated the differences in contrasting genotypes and thus provide valuable information on the pivotal molecular mechanisms underlying salt tolerance mainly in common bean, but for all crops.

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Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach

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