Centromeric R loops contribute to defects in kinetochore assembly and chromosomal instability

ABSTRACT: We mapped ssDNA formation in WT and hpr1delta cells at permissive (30C) and non-permissive (37C) temperature. We showed that hpr1 cells accumulate ssDNA show elevated levels of ssDNA at centromeric regions. Additionally, we show replication fork-associated ssDNA at origins of replication is more confined to the origins in hpr1 cells than WT cells, suggesting a defect in replication fork progression.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE151849 | GEO | 2020/12/31

REPOSITORIES: GEO