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Characterizing gene expression changes within the human hair follicle bulb driven by hepatocyte growth factor secreted by perifollicular fat

ABSTRACT: Dermal white adipose tissue (dWAT) has been linked to modulation of murine hair follicle growth and cycling, however no studies have characterized its effects upon human hair growth. Since HGF was detected as a dWAT secretion product in HF-dWAT co-culture, the aim of this study was to characterize changes in gene expression in human dissected hair follicles vs. follicles cultured with surrounding dermal fat treated with/without a neutralizing antibody to HGF. To this end, the hair matrix and pre-cortex in each condition was isolated using laser-capture microdissection and microarray was carried out on the amplified cDNA. We identified 593 genes differentially modulated owing to the dWAT-derived HGF i.e. 301 up-regulated genes and 292 down-regulated genes in the presence of dWAT-secreted HGF and vice versa when HGF is neutralized.

ORGANISM(S): Homo sapiens

PROVIDER: GSE151972 | GEO | 2023/05/29

REPOSITORIES: GEO

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Profiling the response of scalp hair follicles to short-term treatment with hepatocyte growth factor

Project description:Hepatocyte growth factor (HGF) is a well-known promoter of cell proliferation, morphogenesis, migration, angiogenesis and pigmentation. To characterize its effects on human scalp dissected hair follicles, we performed gene expression profiling on RNA isolated from entire hair follicles, comparing the effects of 6hr HGF treatment versus control. 183 genes were differentially up-/down-regulated compared to vehicle control. These genes were involved in a number of different biological pathways suggesting that HGF can have various effects upon human hair follicle function.

2023-05-29 | GSE151560 | GEO

SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells

Project description:Global expression analysis of neural crest-like skin-derived precursors (SKPs) and Sox2-positive follicle dermal cells that SKPs originate from. In spite of the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we provide evidence that SKPs, multipotent neural crest-like skin-derived precursors, represent an adult dermal stem cell. When transplanted into adult skin, SKPs can reconstitute the adult dermis, contribute to dermal wound-healing, home to a hair follicle niche, and instruct epidermal cells to make hair follicles. Hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. The endogenous origin of SKPs are Sox2-positive follicle dermal cells that share a similar global gene expression profile with SKPs. These endogenous cells home back to their follicle niche, induce hair follicle morphogenesis, and differentiate into neural and dermal progeny. Hair follicle-associated dermal cells will move out of their niche to contribute to dermal maintenance and wound-healing. These studies therefore identify a dermal stem cell, and provide a biological rationale for the presence of a multipotent precursor within adult dermis, findings with important therapeutic implications.

2009-10-23 | GSE18690 | GEO

Isolated of dermal papilla cells from follicular unit extraction

Project description:Hair follicles were originally obtained in the form of intact hair follicles from human scalp during microscopic hair transplant procedures of Follicular Unit Extraction (FUE). Dermal papilla cells were freshly isolated or traditionally isolated and cultured in basic medium supplied with 10% FBS. Freshly-isolated DPCs and cultured DPCs were sequenced using third generations of sequencing.

2022-12-01 | GSE142095 | GEO

Dynamic networks of lymphatic vessels interconnect nodes of neighboring hair follicles across the skin

Project description:Dermal lymphatics form a network that connects all the hair follicles in skin and localize in proximity to the Hair Follicle Stem Cell. RNA sequencing analyses of isolated dermal lymphatics at two different time points of the hair follicle cycle (P55 and P70) indicate the existence of dynamic signaling networks associated with lymphatic remodeling, immune trafficking, and HF signaling.

2019-01-01 | GSE102463 | GEO

Gene expression profiling of the intact dermal sheath cup of human hair follicle

Project description:The dermal sheath cup is the peribulbar component of the hair follicle dermal sheath, and has hair inductive potential similar to the dermal papilla. To characterize it in comparison with other mesenchymal follicle tissuesparts, we performed gene expression profiling of intact dermal sheath cups, which were separated from hair follicles by microdissection. Gene expression profiles of the dermal sheath cup, dermal papilla and upper dermal sheath were compared. We identified a dermal sheath cup signature composed of 32 upregulated genes, which included extracellular matrix components and BMP binding mollecules, while dermal papilla signature included a number of dermal papilla signature genes which had already reported. Analyses of upstream regulators showed that TGF- b1 is a putative regulator of these genes. These results suggest some of molecular mechanism that contributes to human dermal sheath cup properties, which could be useful for hair follicle bioengineering.

2020-02-22 | GSE95219 | GEO

Transcripotome analysis of different locations of hair follicles (bulb, bulge) in androgenetic alopecia

Project description:To fully interrogate location-specific variations of the hair follicle in AGA and normal persons, we sought to perform a thorough and comprehensive transcripotome profiling of different locations of hair follicles (bulb portions, bulge portions) in balding and non-balding areas of AGA, with normal comparisons.

2017-12-25 | GSE101451 | GEO

Next-generation sequencing of microRNAs in different locations of hair follicles (bulb, bugle)in androgenetic alopecia

Project description:To fully interrogate location-specific variations of microRNAs of the hair follicle in AGA and normal persons, we sought to perform a thorough and comprehensive microRNA profiling of different locations of hair follicles (bulb portions, bugle portions)

2018-12-24 | GSE111788 | GEO

Transcriptome analysis of cells in mouse hairy melanocytic nevi

Project description:RNA-seq data was obtained from different skin cell types at two time points: postnatal day P30, when WT dorsal hair follicles are in actively growing state (aka anagen) and P56, when WT hair follicles are in resting state (aka telogen). Cell types include: Bulge stem cells (B), dermal papilla fibroblasts (DP), secondary hair germ cells (HG), melanocytes (Melan), myeloid cells (Myel) and CD45-positive immune cells (I). All cell were FACS sorted and only live single cells were collected for RNA extraction.

2023-03-14 | GSE111999 | GEO

Comparative gene expression analysis of 2 subpopulations of dermal papilla cells.

Project description:Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue. We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. The Sox2-positive CD133-positive cells are found exclusively in the dermal papillae of guard/awl/auchene hairs, while Sox2-negative, CD133-positive cells are found in the other hair follicle types. We compared these populations with unfractionated dermal cells. We isolated the following 3 populations of cells from the back skin of neonatal mice (P2) by Flow Cytometry: 1) GFP-CD133- Total dermal cells 2) GFP-CD133+ Dermal Papilla cells 3) GFP+CD133+ Dermal Papilla cells The yield is approximately 50,000 cells of each population.

2009-06-26 | E-GEOD-16801 | biostudies-arrayexpress

Transcription profiling of Gata3 conditional knock out mice to investigate epidermis and hair follicle differentiation

Project description:Transcriptome analysis on Gata3 conditional knock out murine hair follicles. Background. The transcription factor Gata3 is critically involved in epidermis and hair follicle differentiation. Yet, little is known about how Gata3 co-ordinates stem cell lineage determination in skin, which processes are mostly implicated and how Gata3 differentially regulates distinct cell populations within the hair follicle. Here, we describe a conditional Gata3-/- mouse (K14-Gata3-/-) in which Gata3 is specifically deleted in epidermis and hair follicles. Principal findings. K14-Gata3-/- mice show aberrant postnatal growth and development, delayed hair growth and maintenance, abnormal hair follicle organization and irregular pigmentation. After the first hair cycle, the germinative layer surrounding the dermal papilla was not restored; instead, proliferation was pronounced in basal epidermal cells. Transcriptome analysis of laser-dissected K14-Gata3-/- hair follicles as compared to wild type littermate controls, revealed mitosis, epithelial differentiation and the Notch, WNT and BMP signalling pathways to comprise significantly overrepresented processes. Conclusions. Subsequent elucidation of these pathways at the RNA and protein levels and physiologic endpoints shows that Gata3 integrates diverse signalling networks to regulate the balance between hair follicle and epidermal cell fates.

2007-01-01 | E-MEXP-634 | biostudies-arrayexpress

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