Transcriptomics,Genomics

Dataset Information

22

Biocompatibility and Discovery of the Potential Applications of Magnetite (Fe3O4) Nanoparticles


ABSTRACT: A Transcriptomics Approach to Study the Biocompatibility and Finding out the Potential Applications of Magnetite (Fe3O4) Nanoparticles Here in this study, we examine the molecular effects of uptake of Fe3O4 nanoparticles using a whole genome microarray study in human epithelial cancer cell line. 38 genes (55%) out of 69 downregulated genes were found to be associated with TGF-beta or BMP signaling including six genes, Id1, Id2, Id3, Caspase-9, Smad6 and SMAD7, important negative regulators of these signaling pathways involved in development and tumorigenesis. Overall design: Total RNA isolated from MNP treated HeLa cells compared to untreated controls.

INSTRUMENT(S): Illumina human-6 v2.0 expression beadchip

SUBMITTER: Jameel Ahmad Khan  

PROVIDER: GSE15248 | GEO | 2010-03-13

SECONDARY ACCESSION(S): PRJNA116539

REPOSITORIES: GEO

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Publications

Magnetite (Fe3O4) nanocrystals affect the expression of genes involved in the TGF-beta signalling pathway.

Khan Jameel Ahmad JA   Mandal Tarun Kumar TK   Das Taposh Kumar TK   Singh Yogendra Y   Pillai Beena B   Maiti Souvik S  

Molecular bioSystems 20110219 5


An understanding of interaction of nanomaterials with living systems is fundamental to address nanosafety issues, which, in turn will dictate the future prospects of nanomedicine. Herein, we examine the molecular effects of uptake of Magnetite (Fe(3)O(4)) Nanocrystals (MNC) using a transcriptomics approach. The uptake of MNC was studied by electron microscopy. This was followed by transcriptional profiling using whole genome microarrays, functional analysis of microarray data, real time PCR and  ...[more]

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