Effect of microRNA-27b-3p mimic treatment on human osteoarthritis fibroblast-like synoviocyte gene expression profiles

ABSTRACT: OBJECTIVE: To identify the changes in gene expression elicited by miR-27b-3p mimic transfection of human osteoarthritis (OA) primary fibroblast-like synoviocytes (FLS) obtained from patients undergoing knee arthroplasty. Cells were cultured, and treated for 48 h with miR-27b-3p mimic or Cel-miR-39-3p control mimic. Total RNA was then extracted, and mRNA libraries were prepared using Illumina's TruSEQ stranded total RNA library preparation kit, pooled together, and sequenced on Illumina's NextSeq550.

ORGANISM(S): Homo sapiens

PROVIDER: GSE152638 | GEO | 2022/08/30

REPOSITORIES: GEO