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Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

ABSTRACT: Mulberry (Morus atropurpurea) is an important economic woody tree with rapid growth rate and large biomass, which had great potential for heavy metals remediation. To further understand the mechanisms involved in cadmium accumulation and detoxification in mulberry, we carried out a transcriptomic study to get insights into the molecular mechanisms of the mulberry response to cadmium stress using RNA-seq analysis with BGISEQ-500.

ORGANISM(S): Morus alba var. atropurpurea

PROVIDER: GSE152672 | GEO | 2022/06/30

REPOSITORIES: GEO

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Effects of Morus alba L. (Mulberry) Leaf Extract in Hypercholesterolemic Mice on Suppression of Cholesterol Synthesis

Project description:To investigate effects of intake of mulberry leaf extracts on hypercholesterolemia, we performed gene expression profiling on rat liver by microarray analysis. Microarray analysis revealed that mulberry leaf extracts up-regulated the gene expression involved in suppression of cholesterol synthesis and stimulation of innate-adaptive Immunity. Mice were fed a high-cholesterol diet without/with orally administration of mulberry leaf extracts for 4 weeks. Livers were taken for RNA extraction and hybridization on Agilent microarrays.

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Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

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Project description:Paper mulberry as a valuable woody species has a well chilling tolerance. In this study, phosphoproteomic analysis in combination with physiological measurement and mRNA quantification were employed to explore the molecular mechanism of chilling (4 °C) tolerance in paper mulberry. After chilling for 6 hours, there were 427 significant changed phosphoproteins detected in paper mulberry seedlings without obvious physiological injury. When obvious physiological injury occurred after chilling for 48 hours, a total of 611 phosphoproteins were found significantly change at phosphorylation level. According to 9 phosphorylation motifs extracted by Motif-X analysis, MAPKs, CDPKs, CDKs and CKs were considered as the primary upstream protein kinases. Results of GO analysis showed that phosphoproteins were mainly responsible for signal transduction, protein modification and translation during chilling. Additionally, transport and cellular component organization were respectively enriched after chilling for 6 and 48 hours. Based on the analysis of protein-protein interaction network, a protein kinases and phosphatases hub protein was thought as the key of phosphorylation regulation, which probably modulates cross-talk between Ca2+, BR, ABA and ethylene mediated signaling pathways. Together with results, we concluded a schematic chilling tolerance mechanism at phosphorylation level.

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Identification and Comparative Analysis of Drought Responsive microRNA Targets in Mulberry (Morus alba L.) by Degradome Sequencing

Project description:MicroRNAs (miRNAs) are endogenous non-coding small RNAs containing 21-24 nucleotides, and play an important function in the course of stand up to adversity. Identification of miRNA target mRNAs is essential for their functional analysis. In order to anatomize miRNA guided gene regulation under drought stress, we performed a transcriptome-wide experimental method using high throughput degradome sequencing to directly detect drought stress responsive miRNA targets in mulberry. In this study, drought library (DL) and contrast library (CL) which captured the cleaved mRNA were constructed from mulberry for sequencing by Illumina sequencer, and 409, 373 target genes for 30 conserved miRNA families and 990, 950 target genes for 199, 195 novel miRNAs were identified in CL and DL, respectively. Among those conserved miRNA families in DL, mno-miR156, mno-miR172 and mno-miR396 refer to the highest number of targets, indicating that mno-miR156, mno-miR172 and mno-miR396 families and their target genes might play a key important function in corresponding to drought stress in mulberry. By construction and analysis of miRNA-target network, we found that the DL independent networks consist of 838 miRNA-mRNA pairs (63.34%). Besides, the expression patterns of 11 target genes and 12 correspondent miRNAs were compared and analyzed by qRT-PCR. In addition, 5 of 6 miRNA targets were further verified by RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-5’ RACE). Gene ontology (GO) annotations and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis showed that these target transcripts were implicated in a broad range of biological processes and various metabolic pathways. The characterization of target genes and the associated miRNAs in response to drought exposure provides a framework for understanding the molecular mechanism of resistance to drought in plants.

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