Unknown

Dataset Information

25

Analysis of fetal livers from male mice after protein-restricted diet in mothers


ABSTRACT: Prenatal nutrition as influenced by nutritional status of the mother has been identified as a determinant of adult disease. Feeding low-protein diets during pregnancy in rodents is a well-established model to induce “programming” events in offspring. We hypothesized that protein restriction would influence fetal lipid metabolism by inducing epigenetic adaptations. Methodology/Principal Findings: Pregnant C57BL/6J OlaHsd mice were exposed to a protein restriction protocol (9% vs. 18% casein). Shortly before birth, dams and fetuses were sacrificed. To identify putative epigenetic changes, CpG island methylation microarrays were performed on DNA isolated from fetal livers. Overall design: 10 fetal livers: 5 mice from protein-restriction group (mice 1-5, labeled with Alexa 647) and 5 mice from control group (mice 6-10, labeled with Alexa 555). 5 arrays were used for the mice combinations 1/6, 2/7, 3/8, 4/9, 5/10. All arrays were run at the UHN Microarray Centre, Toronto.

INSTRUMENT(S): MCGI4.6Kv1_CPG_Mouse_Array_UMCG

ORGANISM(S): Mus musculus  

SUBMITTER: vincent bloks  

PROVIDER: GSE15280 | GEO | 2009-11-03

SECONDARY ACCESSION(S): PRJNA116505

REPOSITORIES: GEO

Similar Datasets

2009-11-02 | E-GEOD-15280 | ArrayExpress
2015-09-07 | PXD002578 | Pride
2009-08-06 | GSE15146 | GEO
2011-01-14 | E-GEOD-25898 | ArrayExpress
2011-01-14 | GSE25898 | GEO
| GSE105772 | GEO
| GSE50034 | GEO
2013-07-29 | E-GEOD-49291 | ArrayExpress
| PRJNA116505 | ENA
2010-09-30 | E-GEOD-23106 | ArrayExpress