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Differences in Gene Expression Profiles between the chorion-attached and non-chorion-attached endometrium at the mid-gestation of female rabbit

ABSTRACT: The placenta, the complex of fetal membrane (the chorion is attached to the endometrium) and endometrium, is a highly specialized temporary organ responsible for the normal progression of pregnancy in mammals. Therefore, the endometrium was divided into two function regions: chorion-attached (CA) and non-chorion-attached (NCA) region. However, the difference in gene expression profiles between the CA and NCA endometrial tissue of mid-gestation still are unknown. The objective of this study was to reveal gene expressed profiles of the CA and NCA endometrium from the mid-gestational female rabbits, and to screen the differentially expressed genes (DEGs) to imply major physiological events of endometrial at mid-gestation. RNA-seq showed that a total of 12320 genes were co-expressed in the CA and NCA endometrium, and 646 DEGs were identified. Enrichment analysis of the DEGs revealed the top overrepresented gene ontology (GO) term and pathways were related to immune regulation, cellular adhesions. Furthermore, the expression levels of DEGs related to immune regulation of chemokines and their receptors, extracellular matrix (ECM) and Integrin, were compared between the CA and NCA endometrium. Overall, there was hardly difference in the expression levels of immune regulation genes (chemokines and their receptors) between the CA and NCA endometrium, while the concentration of IL-8, IL-6 and IL-1β in the CA endometrium was higher than that in the serum and the NCA endometrium, which suggested immune cytokines were accumulated in the endometrium of maternal-fetal interface. Meanwhile, the expression level of genes related to cellular adhesions (ECM-integrin) in the NCA endometrium was significantly higher than that in the CA endometrium, and high abundance of Integrin β and THBS1 were localized in the luminal epithelium of the NCA endometrium, but not in the CA endometrium. Conclusions: Our study revealed the gene expression profiles of the CA and the NCA endometrium at mid-gestation, and provides an implication of chemokine and ECM-Integrin involved in immune regulation and adhesive barrier at the endometrium.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE152905 | GEO | 2020/06/23

REPOSITORIES: GEO

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Project description:The fibroblast-populated 3D collagen matrix has been used to study the effect of mechanical stress on cell fate; this process is relevant to the fields of wound healing and tissue engineering. Gene array data was generated from mechanically stressed vs. stress-released matrices. The parameters of the collagen matrix model were: collagen type = bovine type I; collagen concentration = 1.5 mg/mL; initial matrix volume = 0.2 mL; initial matrix diameter = 11 mm (cultured in 24-well plates); cell type = human foreskin fibroblast, passage <10; initial matrix cell concentration = 1,000,000 cell/mL (200,000 cell/matrix); culture medium = 5% FBS in DMEM with 1 M-BM-5g/mL ascorbate. Matrices (n = 6 per experimental group) were incubated for 24 hr in the attached state; the released groups then underwent matrix detachment from the culture plate (defined as t = 0), while the attached groups were left undisturbed. RNA was isolated from attached and released matrices 6 and 24 hr after t = 0. Gene expression in the attached vs. released condition at 6 or 24 hr then was analyzed by hybridizing the anti-sense RNA derived from attached and released matrices at a given time point onto a single chip. Refer to the attached Figure 1 for the experimental design. The index experiment was defined as the comparison of gene expression in attached vs. released collagen matrices in a single strain of human foreskin fibroblasts at 6 and 24 hr after stress-release (i.e., after t = 0). Each experiment utilized two mechanical conditions (attached and released) at two time points (6 and 24 hr). So with each condition utilizing 6 matrices, each index experiment required a total of 24 matrices. In each index experiment, the chip hybridizations were: (i) 6 hr attached vs. 6 hr released, and (ii) 24 hr attached vs. 24 hr released (i.e., two gene chips per index experiment). Each hybridization was done using a 10K spotted gene chip manufactured in the UNMC Microarray Core Facility. The index experiment was performed on three fibroblast strains, meaning that expressional data was derived from three foreskin donors (nonpooled samples). Dye-swap was not performed; dye assigned to attached vs. released remained constant among all chips. Since the index experiment was performed three times, the total number of gene chips used for this entire dataset was six.

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Differences in Gene Expression Profiles between the chorion-attached and non-chorion-attached endometrium at the mid-gestation of female rabbit

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