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10x Genomics single-cell RNA-seq of isolated Adult ID4-EGFP-bright mouse spermatogonia treated with Rapamycin

ABSTRACT: To reveal distinct transcriptome changes among ID4-EGFP-bright adult mouse spermatogonia associated with mTORC1 activity, single-cell transcriptomes were generated from GFP-bright/CD9-bright spermatogonia from adult mice in three groups: control (untreated), 2 days of Rapamycin treatment (Rapamycin) and 2 days Rapamycin plus 1 day washout (Rapamycin_Release). Based on transplantation studies performed previously, ID4-EGFPbright cells are highly enriched for SSCs. We used the 10x Genomics Chromium to perform single-cell RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE152930 | GEO | 2021/02/18

REPOSITORIES: GEO

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