Bone marrow stromal cells induce an ALDH+ stem cell-like phenotype and enhance chemotherapy resistance in AML cells through a TGF-β-p38-ALDH2 pathway

ABSTRACT: Our study aims to examine the interaction between AML cells and other cells in the tumor microenvironment, specifically bone marrow derived mesenchymal stromal cells. To delineate the specific genes/pathways involved in this interaction, we have co-cultured AML cell lines alone or with bone marrow derived mesenchymal stromal cells for 3 days, extracted total RNA from AML cells and analyzed the samples by RNA-seq. We identified the genes that are differentially expressed in co-cultured AML cells compared to AML cells alone. We found that TGF-β1 associated gene signature is activated in AML cells in the presence of BM-MSCs compared to AML cells alone.

ORGANISM(S): Homo sapiens

PROVIDER: GSE152996 | GEO | 2020/06/23

REPOSITORIES: GEO