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N6-methyladenosine (m6A) methylated RNA immunoprecipatation and sequencing in wildtype and Mettl5-/- embryonic stem cells.

ABSTRACT: We report the application of methylated RNA immunoprecipatation and sequencing technology for high-throughput profiling of m6A modifications in embryonic stem cells (ESCs). By using proteinA/G and m6A antibody, we obtained immunoprecipitated RNA of mouse ESCs. RNAs can be read in a single nucleotide accuracy analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE153139 | GEO | 2020/10/01

REPOSITORIES: GEO

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Mapping N6-methyladenosine (m6A) peaks in wild-type and METTL5 knockout (KO) HeLa cells

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