Genomics

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Sox2 Levels Define Pluripotent Ground States and Reprogramming Potential


ABSTRACT: Pluripotent stem cell lines derived from embryos of different stages have distinct pluripotent ground states, but similar levels of the transcription factor Oct4. Epiblast-derived pluripotent stem cells (EpiSCs), in contrast to embryonic stem (ES) cells, cannot form chimeras. We show that EpiSCs express lower levels of the transcription factors Sox2 and Klf4 than ES cells and have limited reprogramming potential, as shown by cell fusion. Sox2 overexpression dramatically increases the reprogramming potential, chimera formation, and germline contribution of EpiSCs. Therefore, although Oct4 is essential for reprogramming, the level of Sox2 defines both the reprogramming capability and the pluripotent ground states.

ORGANISM(S): Mus musculus

PROVIDER: GSE15358 | GEO | 2010/12/31

SECONDARY ACCESSION(S): PRJNA115777

REPOSITORIES: GEO

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