Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide CRISPRi screen for myriocin sensitivity

ABSTRACT: To delineate cellular pathways underlying cell growth in the absence of sphingolipid syntehsis, we performed a genome-wide CRISPRi screen to identify genetic modifiers of myriocin sensitivity in human K562 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE154045 | GEO | 2020/07/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide CRISPRi screen for myriocin sensitivity

Project description:Genome-wide CRISPRi screen for myriocin sensitivity

| PRJNA644921 | ENA

Gene expression by myriocin treatment

Project description:Studies of aging and longevity are revealing how diseases that shorten life can be controlled to improve the quality of life and lifespan itself. Two strategies under intense study to accomplish this goal are rapamycin treatment and calorie restriction. New strategies are being discovered including one that uses low-dose myriocin treatment. Myriocin inhibits the first enzyme in sphingolipid synthesis in all eukaryotes and we showed recently that low-dose myriocin treatment increases yeast lifespan at least in part by down-regulating the sphingolipid-controlled Pkh1/2-Sch9 (ortholog of mammalian S6 kinase) signaling pathway. Here we show that myriocin treatment has global influences and modulates the evolutionarily conserved Snf1/AMPK, PKA and TORC1 signaling pathways to enhance yeast lifespan. These extensive affects of myriocin rival those of rapamycin and calorie restriction. Our studies in yeast along with other studies in mammals reveal the potential of myriocin or related compounds to lower the incidence of age-related diseases in humans. No-myriocin-treated cells and myriocin-treated cells; three biological replicates in each treatment

2014-09-22 | E-GEOD-41362 | biostudies-arrayexpress

Gene expression by myriocin treatment

2014-09-22 | GSE41362 | GEO

Skeletal muscle of aged mice, and aged treated with myriocin

Project description:To investigate the effect of intraperitoneal myriocin treament on skeletal muscle gene expression in aged mice.

2022-12-21 | GSE213110 | GEO

In vitro antifungal activity of myriocin against drug-resistant Candida auris strains

Project description:Myriocin exhibited very low MIC values (0.016 to 0.4 mg/L) to all C. auris isolates including several multidrug-resistant strains. The yeast-form, filamentous, or aggregating-form cells were all susceptible to the chemical. The antifungal effect was majorly due to the fungistatic activity, although an extended treatment could lead to the fungicidal activity to C. auris cells. Transcriptomic analysis demonstrates that myriocin suppresses the cellular metabolic activity and induces the expression of stress or antifungal response genes.

2020-12-03 | GSE161599 | GEO

Pooled CRISPRi screen with scRNA-seq to knockdown 7 transcription factors in k562 cells

Project description:To systematically investigate the gene regulatory networks in K562 cells, we performed CROP-seq—a pooled CRISPRi screen combined with single-cell RNA-seq—targeting seven transcription factors. To enhance gRNA recovery, we incorporated the Single Cell 5′ CRISPR Screening Assay, which enables direct capture of gRNA sequences on the beads and generates a gRNA count matrix.

2026-02-11 | GSE308682 | GEO

CRISPRi screen of prostate cancer associated risk cis-regulatory elements [CRISPRi]

Project description:CRISPRi screen of prostate cancer associated risk cis-regulatory elements [CRISPRi]

| PRJNA598432 | ENA

CRISPRi/a screen of doxorubicin induced cardiotoxicity

Project description:Druggable genome of CRISPRi/a sgRNA libraries for druggable genes protecting doxorubicin induced cardiomyocyte toxicity using human induced pluripotent stem cell (iPSC) derived cardiomyocytes.

2024-09-01 | GSE276161 | GEO

RNA-seq of CSMD1 enhancer CRISPRi in LUHMES cells and differentiated neurons

Project description:we performed lentiviral CRISPR interference (CRISPRi) by recruiting dCas9 fused with the KRAB domain to the CSMD1 enhancer (fam3) in the neuronal precursor cell line – Lund human mesencephalic (LUHMES). Given that the expression of CSMD1 was not detectable in LUHMES cells we differentiated these cells into neurons. Differentiated neurons with CRISPRi of CSMD1 enhancer showed significantly higher expression of CSMD1 than control.

2022-06-30 | E-MTAB-8027 | biostudies-arrayexpress

Effects of CYTOR Knockdown via CRISPRi on K562 cells

Project description:Effects of CYTOR Knockdown via CRISPRi on K562 cells

| PRJNA1201158 | ENA