Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic profiling of fetal human hepatocytes immortalized with hTERT

ABSTRACT: The successfully immortalized fetal human hepatocytes expressed large numbers of hepatic and other genes. The expression of gene groups and networks was maintained over extended culture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE154198 | GEO | 2020/07/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genetic profiling of fetal human hepatocytes immortalized with hTERT

Project description:Genetic profiling of fetal human hepatocytes immortalized with hTERT

| PRJNA645400 | ENA

Comparison of primary vs immortalized murine hepatocytes

Project description:Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors and species. Cell type specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research and tailored drug development. In these experiments primary murine hepatocytes were compared to immortalized murine hepatocytes with respect to their global gene expression pattern.

2018-02-03 | GSE110065 | GEO

Comparison of primary vs immortalized murine hepatocytes

Project description:Comparison of primary vs immortalized murine hepatocytes

| PRJNA432701 | ENA

Transcriptomic Profiles of Immortalized Human Hepatocytes Overexpressing Intracellular Domain of NOTCH1

Project description:To investigate the effect of hyperactivation of Notch signaling in hepatocytes, an immortalized human hepatocyte cell line THLE-2 was stably transduced with control vector or plasmid harborning intracellular domain of NOTCH1 (NICD1) and the transcriptomic profiles were analyzed by RNA-seq.

2019-10-01 | GSE121170 | GEO

Transcriptomic Profiles of Immortalized Human Hepatocytes Overexpressing Intracellular Domain of NOTCH1

Project description:Transcriptomic Profiles of Immortalized Human Hepatocytes Overexpressing Intracellular Domain of NOTCH1

| PRJNA495931 | ENA

Metformin in fetal hepatocytes

Project description:Fetal sheep hepatocytes were treated for 24h with metformin

2023-02-07 | GSE224387 | GEO

Establishment of hTERT immortalized mouse cardiosphere derived cell line

Project description:The analysis showed differences of transcriptomes between primary CDCs and immortalized CDCs as well as revealed TGF-β inhibitor-SB431542 treatment induced expression changes. We identified hTERT immortalization could result the cell proliferation and cell cycle related genes upregulation in iCDC. KEGG results demonstrated that TGF-β inhibitor SB431542 treatment could inhibit cardiomyopathy-related pathway which indicate TGF-β signaling pathway may serve as new target for cardiomyopathy therapy, and iCDC could be used as a heart-related chemicals screening.

2019-12-31 | GSE131765 | GEO

Expression data for hTERT-immortalized human Sertoli cell line versus original primary human Sertoli cells

Project description:Primary human Sertoli cells (hSerCs) were immortalized by transducing human TERT into the genome to produce human TERT-immortalized human Sertoli cells (hTERT hSerCs). Total RNA was isolated from both primary hSerCs and hTERT hSerCs and submitted for Affymetrix gene analysis. Gene expression differences of common Sertoli cell marker genes and xenobiotic transporters was preferentially assessed between each cell type.

2020-09-23 | GSE158400 | GEO

Comparison of the transcriptomes of hTERT-immortalized and wild type marmoset fibroblasts by next-generation RNA sequencing.

Project description:Neuborn marmoset fibroblasts were immortalized by piggyBac transposition of hTERT expression cassette. The gene expression of the resulting one polyclonal and two monoclonal immortalized cell lines were then compared with the wild type cells used for their derivation.

2018-10-29 | GSE114965 | GEO

Establishment of hTERT immortalized mouse cardiosphere derived cell line.

Project description:Establishment of hTERT immortalized mouse cardiosphere derived cell line.

| PRJNA669880 | ENA