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Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling [bulk RNA-seq]

ABSTRACT: We performed bulk RNA-seq on pancreatic and intestinal tuft cells, bone marrow derived macrophages with various treatments, and mouse pancreatic stellate cells with various treatments.

ORGANISM(S): Mus musculus

PROVIDER: GSE154258 | GEO | 2020/07/13

REPOSITORIES: GEO

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Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling [single-cell RNA-seq]

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Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling

Project description:Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling

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Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling [bulk RNA-seq]

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Tuft cells play a suppressive role in pancreatic injury and tumorigenesis [RNA-seq]

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Lgr5+tuft marker+ intestinal tumor cells

Project description:We previously identified Dclk1, a tuft cell marker, marks tumor stem cells (TSCs) in mouse intestinal tumors. In this study, we have identified IL17RB as a cell surface marker distinctively expressed by Dclk1+ tuft-like tumor cells in mouse intestinal tumors. Using this tuft cell marker, we compared and analyzed the transcriptome of Lgr5-tuft marker-, Lgr5+tuft marker-, Lgr5-tuft marker+, and Lgr5+tuft marker+ tumor cells. These analyses revealed that tuft-like tumor cells in the intestinal tumors comprise two distinct subsets: highly differentiated tuft-like tumor cells (Lgr5-tuft marker+ cells) and tuft-like tumor cells with TCS potential (Lgr5+tuft marker+ cells).

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