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The beating cluster of adipose tissue-derived stromal cells

ABSTRACT: Adipose tissue-derived stromal vascular fraction (Ad-SVF) contains stem cells and rarely transdifferentiate into spontaneously beating cardiomyocyte-like cells (beating CMs). However, the factors that regulate the differentiation of Ad-SVF into the cardiac lineage is unknown. We developed a simple culture protocol under which the adult murine inguinal Ad-SVF reproducibly transdifferentiates into beating CMs without induction. We used microarray analyses to evaluate the global gene expressions of the beating CMs and investigated the mechanism underlying the transdifferentiation of SVF cells into CMs.

ORGANISM(S): Mus musculus

PROVIDER: GSE154301 | GEO | 2021/01/21

REPOSITORIES: GEO

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Project description:Introduction: The subcutaneous adipose-tissue-derived stromal vascular fraction (Sa-SVF) contains stem/progenitor cells that rarely transdifferentiate into beating cardiomyocytes. Previously, we developed a culture protocol in which adult murine inguinal Sa-SVF cells reproducibly transdifferentiate into beating cardiomyocyte-like cells (beating CMs) without any specific induction in the primary culture. However, the mechanism underlying the differentiation of the Sa-SVF toward the cardiac lineage is unclear. Methods: To identify a key regulator of cardiac differentiation, we investigated sequential changes in global gene expression profiles of Sa-SVF cells during primary culture. Sa-SVF cells were isolated from adult murine inguinal subcutaneous fat pads and cultured using our beating CM induction method. At six time points during primary culture, total RNA was extracted and subjected to RNA-sequencing and quantitative polymerase chain reaction analysis. Results: Beating CMs appeared on day 14. Of 14,574 prefiltered genes, 749 showed significant expression changes (153 upregulated and 596 downregulated, > 2-fold) between days 7 and 14, coinciding with the appearance of beating CMs. Gene ontology analysis highlighted histone deacetylase (HDAC)-associated genes, expression of which was transiently suppressed during the period when cardiac sarcomere-related genes and the transcription factor Mef2c were upregulated. Treatment with an HDAC inhibitor increased cardiac troponin T expression on day 28. Furthermore, treatment of Mef2c-transduced Sa-SVF cells with an HDAC inhibitor markedly augmented cardiac troponin T expression (18-fold vs. control). Conclusions: We identified transient epigenetic modulation—particularly suppression of class II HDACs—as a key driver of Sa-SVF transdifferentiation into cardiomyocytes. These findings provide a mechanistic basis and a potential combinational strategy to enhance SVF-based cardiac regenerative approaches.

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The beating cluster of adipose tissue-derived stromal cells

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