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Identifying improved sites for heterologous gene integration using ATAC-seq

ABSTRACT: We analyzed the chromatin accessibility and nucleosome positioning by ATAC-seq of both null and transgene-expressing strains of Komagataella phaffii (Pichia pastoris) under different growth conditions. These data enabled identification of the features that determine performance of various integration sites for transgene expression. Understanding chromatin accessibility and nucleosome positioning can provide further clarity into gene regulation and expression broadly in this organism.

ORGANISM(S): Komagataella phaffii

PROVIDER: GSE154330 | GEO | 2020/10/02

REPOSITORIES: GEO

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