Immunological properties of retinal ganglion cells derived from human induced pluripotent cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE154544 | GEO | 2020/10/31
REPOSITORIES: GEO
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Project description:Retinal ganglion cells (RGCs) play a critical role in the transmission of visual signals from the retina to the brain required for proper vision. The optic neuropathies, including glaucoma, NAION, LHON, traumatic optic neuropathy, optic pathway glioma result in the reversible and irreversible changes in RGCs, ultimately leading to their death. RGC loss is permanent given that these cells cannot regenerate in mammals. Neuroregenerative strategies for managing optic neuropathies have mainly focused on restoring the lost RGC cell population in a glaucomatous retina. This treatment approach not only aims to halt disease progression, but also to restore the lost vision by replacing the damaged/lost RGCs through RGC transplantation. Significant strides have been made in this field of RGC transplantation and regeneration. As a first step of RGC transplantation, several protocols now exist to differentiate cells into primary RGCs, and several sources of donor RGC have been established including human ES and iPSC-derived RGCs. To achieve functional regeneration, the transplanted RGCs must survive, integrate, and extend their axons and establish connections within the retina and the brain. Of the many factors influencing initial donor RGCs survival, the innate immunity [ref] and primarily microglia reactivity is of particular interest. In ocular models of glaucoma, microglia have been heavily implicated in driving disease progression, promoting a pro-inflammatory environment. In some instances, activated microglia have been directly implicated in RGC loss by mediating phagocytosis of the damaged or injured RGCs. In line with this, our recent work, shows that activated host microglia are detrimental to donor RGCs, presumably via phagocytosis of the donor RGCs. We observed that pre-treatment of donor RGCs with FasL and annexin V to block the exposed phosphatidylserine residues of the stressed donor RGCs prior to transplantation reduced activation of host microglia, and better RGC survival rates post transplantation. Studies like these and others underscore the importance of modulating host microglia reactivity for better transplantation outcomes. To further improve donor RGC survival post transplantation, a better understanding of the host myeloid cell reactivity following donor RGC transplantation is paramount. We and others have previously shown the importance of modulating the retinal microenvironment to promote RGC survival. In the current study, we profiled the transcriptome of the host myeloid cell population (CX3CR1-GFP) using a single cell RNA sequencing approach, to delineate the myeloid cell population most relevant for donor RGC survival post transplantation. A focused analysis on the microglia cell population was performed, given their established role in glaucoma disease progression. In addition to profiling the expression pattern of the known classic microglia disease associated signatures such as ApoE, Spp1, and Lgals, we identified several other genes associated with, and or driving microglia activation, including Cybb, Atf3, Aurka and Kif22. Given the known function of these genes in other disease contexts, we believe that these genes are important for modulating donor RGC survival post transplantation, thus warrant further investigation in this context. Taken together, with reference to our established adult mouse retina cell atlas, we present a comprehensive analysis of the host myeloid cell population, specifically focusing on microglia reactivity status, at a single cell resolution level, providing insights into the regulatory mechanisms that may be important for donor RGC survival and integration into glaucomatous retina post transplantation.
2025-12-12 | GSE285564 | GEO
Project description:Donor Macrophages Modulate Rejection after Heart Transplantation
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Project description:Cellular rejection after heart transplantation imparts significant morbidity and mortality. Current immunosuppressive strategies are imperfect, target recipient T-cells, and have adverse effects. The innate immune response plays an essential role in the recruitment and activation of T-cells. Targeting the donor innate immune response would represent the earliest interventional opportunity within the immune response cascade. There is limited knowledge regarding donor immune cell types and functions in the setting of cardiac transplantation and no current therapeutics exist for targeting these cell populations. Distinct populations of donor and recipient macrophages co-exist within the transplanted heart. Donor CCR2+ macrophages are key mediators of allograft rejection and deletion of MYD88 signaling in donor macrophages is sufficient to suppress rejection and extend allograft survival. This highlights the therapeutic potential of donor heart-based interventions.
2022-06-30 | GSE206801 | GEO
Project description:A prospective study for investigating the T-cell receptor (TCR) repertoire in T-cell mediated rejection (TCMR) after kidney transplantation. The pre-formed donor-reactive TCR repertoire was tracked in blood and tissue of patients after kidney transplantation and characteristics of TCR repertoires from patients experiencing a rejection were compared to patients with no histopathological signs of rejection.
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Project description:Acute rejection in cardiac transplant patients is still a contributing factor to limited survival of the implanted heart. Currently there are no biomarkers in clinical use that can predict, at the time of transplantation, the likelihood of post-transplantation acute rejection, which would be of great importance for personalizing immunosuppressive treatment. Within the Biomarkers in Transplantation initiative, the predictive biomarker discovery focused on data and samples collected before or during transplantation such as: clinical variables, genes and proteins from the recipient, and genes from the donor. Based on this study, the best predictive biomarker panel contains genes from the recipient whole blood and from donor endomyocardial tissue and has an estimated area under the curve of 0.90. This biomarker panel provides clinically relevant prediction power and may help personalize immunosuppressive treatment and frequency of rejection monitoring.
2017-09-29 | GSE33970 | GEO
Project description:The study comprises various components: Samples TD: We aims to screen out different gene expression profile in donor biopsies after revascularization , We aims to predict renal allograft dysfunction early after transplantation. Samples AR, ATN, Tx: We aim to screen out different gene expression profile in acute rejection on the kidney. We aim to screen out different gene expression profile in acute tubular necrosis on the kidney. Results from the various study components can help to diagnose renal allograft dysfunction with different causes by distinct gene expression profile. Keywords: acute rejection, acute tubular necrosis, donor biopsies, renal allograft dysfunction Samples AR1-AR17: This study has been accomplished with 17 patients of acute rejection on the kidney.Technical replicates: 2 replicates Samples ATN1-ATN5: This study has been accomplished with 5 patients of acute tubular necrosis on the kidney. Technical replicates: 2 replicates Samples Tx1-Tx14: This study has been accomplished with 14 patients of stable renal function on the kidney.Tecnical replicates:2 replicates(except Tx12) Samples TD1-TD12: This study has been accomplished with 12 patients of donor tissue with stable function early after transplantation on the kidney.Technical replicates: 2 replicates Samples TD13-TD21: This study has been accomplished with 9 patients of donor tissue with renal dysfunction early after transplantation on the kidney.Technical replicates: 2 replicates
2011-02-02 | E-GEOD-26578 | biostudies-arrayexpress
Project description:Retinal ganglion cells (RGCs) are the sole projection neurons that connect the eye with the brain, and the degeneration of these cells in diseases such as glaucoma results in vision loss or blindness. Similar to other neurons throughout the central nervous system, RGCs are postmitotic and therefore highly dependent upon autophagy to remove damaged proteins or organelles to maintain proper cellular homeostasis. Autophagy deficits have been implicated in multiple neurodegenerative diseases including glaucoma. In addition, a subpopulation of glaucoma patients possesses mutations in the autophagy receptor Optineurin (OPTN) resulting glaucoma within a normal range of intraocular pressure, with the OPTN(E50K) mutation known to induce a severe degeneration. Despite this, our knowledge of how autophagy impairment promotes neurodegeneration within RGCs remains limited. We advanced a human pluripotent stem cell (hPSC) model of RGC neurodegeneration with an underlying OPTN(E50K) mutation to study how autophagy disruption contributes to RGC neurodegeneration. We identified OPTN protein was reduced but accumulated within the somas in RGCs with the OPTN(E50K) mutation, and this accumulation was associated with a decrease in autophagic flux. To rule out the possibilities that the specificity of the antibody incapable to recognize E50K region resulting the reduction of OPTN protein, we performed proteomics analysis at week 2 hPSC-RGCs of post purification and identified 154 downregulated proteins as well as 178 upregulated proteins associated with OPTN(E50K) RGCs compared with isogenic controls. Taken together, we identified proteins that were altered by OPTN(E50K) mutation in RGCs, and which may provide potential mechanisms that contribute to RGC neurodegeneration.
2025-05-06 | PXD033173 | Pride
Project description:Antibody-mediated rejection, caused by antibodies against the donor human leukocyte antigen (HLA) molecules plays a fundamental role in graft rejection in transplantation. Most significant is transplant patients who generate antibodies against the donor HLA-DQ have the highest risk of rejection. In this study, we investigated the role of indirect CD4 T cell epitopes in the formation of donor-specific antibodies (DSA). Using antigen mapping techniques in kidney and heart transplant recipients with HLA-DQ DSA, we identified two polymorphic hotspots in the HLA-DQ gene that generated alloreactive CD4 T cell responses. To study the functional significance of indirect CD4 T cells, we first mapped epitopes recognised by H2-Kb C57Bl6 mice against a skin graft from H2-Kd Balb/c mice. We identified a CD4 T cell epitope, specific for amino acids 287-301 derived from the H2-Kd protein, that generated tetramer-binding Kd287+ CD4 T cells during rejection. Importantly, the transfer of Kd287+ CD4 T cells into T cell-deficient mice was sufficient to drive an antibody response against the donor H2-Kd molecule. Lastly, we found that administration of systemic high-dose donor H2-Kd peptides combined with CTLA4-Ig reduced the formation of Kd287+ CD4 T cells and diminished DSA formation. Together, these findings demonstrate that the identification of donor antigens indicates the potential for inducing donor-specific immune tolerance in transplantation.
2024-09-17 | GSE277075 | GEO
Project description:Complications associated with immunosuppression, specifically nephrotoxicity and infection risk, significantly affect graft and patient survival after kidney transplantation. We previously showed that tolerance could be induced and full donor chimerism, as well as immunosuppression withdrawal, was obtained in highly mismatched allograft recipients using a bioengineered stem cell product (FCRx). Gene expression profiles in renal biopsy samples from tolerance-induced FCRx recipients, paired donor organs before implant, and subjects under standard immunosuppression (SIS) without rejection and with acute rejection were compared. This dataset is part of the TransQST collection.
2018-05-07 | E-GEOD-106675 | biostudies-arrayexpress
Project description:Increased levels of donor-derived cell-free DNA (dd-cfDNA) in recipient plasma have been associated with acute cellular rejection (ACR) after heart transplantation. DNA sequence differences have been used to distinguish between donor and recipient cfDNA but epigenetic differences could also potentially identify dd-cfDNA. This study aimed to assess the feasibility of using ventricle-specific methylation patterns in human cfDNA as an alternative biomarker for ACR in cardiac transplantation.
2020-06-18 | GSE152648 | GEO