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Expression data of the kidneys from Pkd1 miR Tg mice treated with metformin

ABSTRACT: We investigated the effects of metformin treatment on polycystic kidney disease in a mouse model of Pkd1-deficiency. Kidneys are harvested in postnatal day 63 after 28 days of intraperitoneal injection of metformin (150 mg/kg/day) or saline for control. Total RNA was extracted for microarray analysis. Gene expression profiles were different between treatment and control groups.

ORGANISM(S): Mus musculus

PROVIDER: GSE154590 | GEO | 2023/07/15

REPOSITORIES: GEO

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RNA-sequencing for kidneys derived from adult Pkd1 knock-out (KO) mice treated with salsalate for two weeks

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Project description:Autosomal dominant polycystic kidney disease (ADPKD) is an important cause of end stage renal disease for which there is no proven therapy 1. Mutations at PKD1 are the principal cause of disease. The disease begins in utero2 and is slowly progressive but it is not known whether cystogenesis is an ongoing process during adult life. We now show that inactivation of Pkd1 in mice prior to post-natal day 13 results in severely cystic kidneys within three weeks, whereas inactivation at day 14 and later results in cysts only after five months. We found that cellular proliferation was not appreciably higher in cystic specimens than in age-matched controls but that the abrupt change in response to Pkd1 inactivation corresponded with a previously unrecognized brake point during renal growth and significant changes in gene expression. These findings suggest that the effects of Pkd1 inactivation are defined by a developmental switch that signals the end of the terminal renal maturation process. These studies show that Pkd1 regulates tubular morphology in both developing and adult kidney but the pathologic consequences of inactivation are defined by the organ’s developmental status, with important clinical implications for our understanding of disease and our approach to therapy. Keywords: Developmental status comparison

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2012-02-16 | E-GEOD-35831 | biostudies-arrayexpress

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Project description:PKD1 gene and protein study

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