Actvity-dependent profiling of lateral septum neurons following restraint stress
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE154749 | GEO | 2020/08/30
REPOSITORIES: GEO
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Project description:The lateral septum contains a number of molecularly defined cell populations. We used the viral-TRAP protocol (Nectow et al.,2017) to profile lateral septum neurotensin neurons.
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Project description:Actvity-dependent profiling of lateral septum neurons following restraint stress
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Project description:To explore the effects of long-term olanzapine treatment on gene expression in the lateral septum of female mice, we conducted a study using female C57BL/6 mice fed with olanzapine-supplemented chow for 12 weeks. The treated mice exhibited significant weight gain and lipid alterations compared to the control group. Subsequently, RNA-seq was performed on the lateral septum brain regions of both the treated and control mice, followed by gene expression profiling to analyze the data.
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Project description:Molecular profiling of lateral septum neurotensin neurons using viralTRAP
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Project description:Coordinated gene expression changes across the CNS help to produce the mammalian maternal phenotype. Lateral septum (LS) is a brain region critically involved with aspects of maternal care, and we recently examined gene expression of whole septum (LS and medial septum) in selectively bred maternal mice. Here, we expand on the prior study by 1) conducting microarray analysis solely on LS in virgin and postpartum mice, 2) using outbred mice, and 3) evaluating the role of sensory input on gene expression changes. Large scale changes in genes related to neuronal signaling were identified, including nine GABAA receptor subunits (p<0.05). Subunits ?4 and ? were downregulated in maternal LS, likely reflecting a reduction in the extrasynaptic, neurosteroid-sensitive ?4/? containing receptor subtype. Conversely, subunits ? and ? were increased in maternal LS. Sixteen K+ channel related genes showed altered expression, as did dopamine receptors Drd1a and Drd2 (both downregulated), hypocretin receptor 1 (Hcrtr1), kappa opioid receptor 1 (Oprk1), and transient receptor potential channel 4 (Trpc4). Expression of a large number of genes linked to developmental processes or cell differentiation were also altered in postpartum LS, including chemokine (C-X-C) motif ligand 12 (Cxcl12), fatty acid binding protein 7 (Fabp7), plasma membrane proteolipid (Pllp), and suppressor of cytokine signaling 2 (Socs2). Additional genes that are linked to anxiety, such as glutathione reductase (Gsr), exhibited altered expression. Pathway analysis also identified changes in genes related to cyclic nucleotide metabolism, chromatin structure, and the Ras gene family. The sensory presence of pups was found to contribute to the altered expression of a subset of genes across all categories. This study suggests that both large changes in neuronal signaling and the possible terminal differentiation of neuronal and/or glial cells play important roles in producing the maternal state. In total, 18 total RNA samples derived from microdissected lateral septum were used for microarray analysis: 6 samples from lactating maternal mice (maternal), 6 samples from maternal mice that have had their pups removed (pups removed), and 6 samples from age-matched virgin mice (virgin). All samples are biological replicates.
2013-03-26 | E-GEOD-43627 | biostudies-arrayexpress
Project description:Coordinated gene expression changes across the CNS help to produce the mammalian maternal phenotype. Lateral septum (LS) is a brain region critically involved with aspects of maternal care, and we recently examined gene expression of whole septum (LS and medial septum) in selectively bred maternal mice. Here, we expand on the prior study by 1) conducting microarray analysis solely on LS in virgin and postpartum mice, 2) using outbred mice, and 3) evaluating the role of sensory input on gene expression changes. Large scale changes in genes related to neuronal signaling were identified, including nine GABAA receptor subunits (p<0.05). Subunits α4 and δ were downregulated in maternal LS, likely reflecting a reduction in the extrasynaptic, neurosteroid-sensitive α4/δ containing receptor subtype. Conversely, subunits ε and θ were increased in maternal LS. Sixteen K+ channel related genes showed altered expression, as did dopamine receptors Drd1a and Drd2 (both downregulated), hypocretin receptor 1 (Hcrtr1), kappa opioid receptor 1 (Oprk1), and transient receptor potential channel 4 (Trpc4). Expression of a large number of genes linked to developmental processes or cell differentiation were also altered in postpartum LS, including chemokine (C-X-C) motif ligand 12 (Cxcl12), fatty acid binding protein 7 (Fabp7), plasma membrane proteolipid (Pllp), and suppressor of cytokine signaling 2 (Socs2). Additional genes that are linked to anxiety, such as glutathione reductase (Gsr), exhibited altered expression. Pathway analysis also identified changes in genes related to cyclic nucleotide metabolism, chromatin structure, and the Ras gene family. The sensory presence of pups was found to contribute to the altered expression of a subset of genes across all categories. This study suggests that both large changes in neuronal signaling and the possible terminal differentiation of neuronal and/or glial cells play important roles in producing the maternal state.
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Project description:At present, the experimental evidences of epigenetic inheritance caused by stresses are beginning to accumulate in some animal models. However, molecular mechanism and biological meaning underlying these phenomena has not been clearly understood yet. Previously, we showed that Drosophila transcription factor-2 (dATF-2) is involved in heterochromatin formation and that phosphorylation of dATF-2 by various stresses, such as heat shock and osmotic stresses, induces heterochromatin disruption. We also revealed these stress dependent epigenome changes mediated by dATF-2 can be inherited to next generations (Seong et al., 2011). In this study, we established a drosophila model of restraint stress (RS) to analyze the transgenerational effects of strong psychological stress. We observed that Drosophila F1 progenies, derived from RS exposed fathers, showed significant disruption of heterochromatin state and that such effect was not observed in the dATF-2 and MEKK1 mutant background. To reveal trangenerational effects and role of paternal restraint stress, we analyzed gene expression profiles of F1 progenies derived from either RS exposed or control fathers of wild-type and dATF-2 KO father.
2020-04-29 | GSE140950 | GEO
Project description:The transition from the non-maternal to the maternal state is characterized by a variety of CNS alterations that support the care of offspring. The septum (including lateral and medial portions) is a brain region previously linked to various emotional and motivational processes, including maternal care. In this study, we used microarrays (PLIER algorithm) to examine gene expression changes in the septum of postpartum mice and employed gene set enrichment analysis (GSEA) to identify possible regulators of altered gene expression. Genes of interest identified as differentially regulated with microarray analysis were validated with quantitative real-time PCR. We found that fatty acid binding protein 7 (Fabp7) and galanin (Gal) were downregulated, whereas insulin-like growth factor binding protein 3 (Igfbp3) was upregulated in postpartum mice compared to virgin females. These genes were previously found to be differentially regulated in other brain regions during lactation. We also identified altered expression of novel genes not previously linked to maternal behavior, but that could play a role in postpartum processes, including glutamate-ammonia ligase (Glul) and somatostatin receptor 1 (Sstr1) (both upregulated in postpartum). Genes implicated in metabolism, cell differentiation, or proliferation also exhibited altered expression. Unexpectedly, enrichment analysis revealed a high number of microRNAs, transcription factors, or conserved binding sites (177 with corrected P-value <0.05) that were significantly linked to maternal upregulated genes, while none were linked to downregulated genes. MicroRNAs have been linked to placenta and mammary gland development, but this is the first indication they may also play a key role in sculpting the maternal brain. Together, this study provides new insights into genes (along with possible mechanisms for their regulation) that are involved in septum-mediated adaptations during the postpartum period. In total, 12 total RNA samples derived from microdissected lateral septum were used for microarray analysis: 6 samples from virgin mice and 6 samples from age-matched lactating maternal mice. All samples are biological replicates.
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