Genomics

Dataset Information

36

High-throughput sequencing of small RNAs in chicken somite embryos


ABSTRACT: High-throughput sequencing of small RNAs in chicken somite embryos Overall design: Dissected tissue white leghorn chicken embryos was disaggregated and RNA extracted using the miRVana kit (Ambion). Small RNA fraction between 19 and 24 nt was isolated from 15% denaturing polyacrylamide gel and 15 micro gram was ligated to Solexa adaptors (Illumina) without de-phosphorylating and re-phosphorylating. The short RNAs were converted to DNA by RT-PCR following the Illumina protocol.

INSTRUMENT(S): Illumina Genome Analyzer II (Gallus gallus)

ORGANISM(S): Gallus Gallus

SUBMITTER: Helio Pais  

PROVIDER: GSE15513 | GEO | 2009-04-28

SECONDARY ACCESSION(S): PRJNA115677

REPOSITORIES: GEO

Dataset's files

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Action DRS
GSE15513_sequence_annotations.txt.gz Txt
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