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Next generation sequencing analysis of primary rat lung fibroblast, primary rat lung alveolar type 2 cell and a novel 10-4A rat lung cell line transcriptomes

ABSTRACT: Purpose: We report the application of single-molecule-based sequencing technology for high-throughput profiling of a novel 10-4A rat lung fibroblast cell line and compared the transcriptome to freshly isolated primary rat lung fibroblasts and freshly isolated primary rat lung alveolar type 2 cells.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE155743 | GEO | 2020/09/18

REPOSITORIES: GEO

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Next generation sequencing analysis of primary rat lung fibroblast, primary rat lung alveolar type 2 cell and a novel 10-4A rat lung cell line transcriptomes

Project description:Next generation sequencing analysis of primary rat lung fibroblast, primary rat lung alveolar type 2 cell and a novel 10-4A rat lung cell line transcriptomes

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Single cell RNAseq of human primary lung adenocarcinomas [scRNA-seq]

Project description:To study heterogenous cellular function and behaviors, we isolated individual cells from freshly resected and dissociated two human primary lung adenocarcinomas, removed DAPI-positive dead cells, and performed single cell analysis using the 10x Genomics Chromium platform.

2024-01-17 | GSE164983 | GEO

Next Generation Sequencing Analysis of primary rat lung fibroblast transcriptomes

Project description:Purpose: We report the application of single-molecule-based sequencing technology for high-throughput profiling of primary rat lung fibroblast cultured on 3-5 kPa PDMS compared to plastic over a 14-day timecourse.

2022-11-07 | GSE199753 | GEO

Transcriptome studies of marine natural products 4a and 4d on non-small cell lung cancer A549 cells

Project description:Two novel polyketides, ocellatusones A and B (1 and 2), characterized by either an uncommon oxatricyclo[4.2.1.02,4]nonane skeleton (1) or a mesitylene connected dimethylfuran-3(2H)-one nucleus (2), were isolated in racemic forms from the South China Sea sacoglossan Placobranchus ocellatus, together with seven uncommon polypropionates (3, 4a,4d, 5, and 6). Compounds 1 and 2 were further separated by chiral HPLC to their corresponding enantiomers (±)-1 and (±)-2, respectively. The structures, including absolute configurations, of the new compounds, were unambiguously determined by extensive spectroscopic analysis, quantum chemical computation, and/or X-ray diffraction analysis. The new compounds (+)-1, 4a, 4b, 4d exhibited interesting dose dependent cytotoxic effect on human cancer cell lines, including non-small cell lung cancer and acute promyelocytic leukemia, with IC50 values ranging from 6.1 μM to 28.8 μM. A plausible bio-photosynthetic pathway of these isolates was proposed.

2019-06-26 | GSE132500 | GEO

Comparison of rat alveolar epithelial type I cells and type II cells

Project description:Comparison of rat freshly-isolated alveolar epithelial type I cells, freshly-isolated type II cells, and type II cells cultured for 7 days Keywords = rat, alveolar epithelial type I cells, cultured type II cells Keywords: parallel sample

2004-11-01 | GSE1567 | GEO

Profiling of Transcripts in Rat Proximal Tubule

Project description:Freshly isolated rat kidney proximal tubules were subjected for transcript profiling.

2009-01-26 | GSE13668 | GEO

Rat lung cells' gene expression difference compared with that of the adult cells including rat BMC and PEF cells

Project description:Rat somatic lung cell had different gene expression level compared with that of the adult cells including rat bone marrow cells(BMC) and rat primary ear fibroblasts(PEF). This difference gave the rat somatic lung cell unique characteristics which could then be compared with the rat iPS cells by genes comparison to show the gene expression difference between rat somatic lung cells and rat iPS cells.

2009-04-01 | GSE13573 | GEO

Profiling of Transcripts in Rat Kidney Medullary Thick Ascending Limb

Project description:Freshly isolated rat kidney medullary thick ascending limbs were subjected for transcript profiling.

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cell surface glycoproteome liver and lung

Project description:Purpose Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue. Experimental design We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture-specific approach combined with a robust bioinformatics filtering. Results Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte-specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver. Conclusions and clinical relevance We demonstrate the feasibility and the importance of conducting such studies directly in a clinically-relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines.

2015-07-22 | PXD001988 | Pride

The effects of chemokines CXCL1/2/8 on mouse lung fibroblasts

Project description:Our preliminary data show that the primary triple-negative breast tumor-derived chemokines CXCL1/2/8 promote migration and tube formation of mouse lung endothelial cells, which is mediated by lung fibroblasts. To explore the molecular events underlying this mediator function of lung fibroblasts, we treated freshly-isolated lung fibroblasts with chemokines CXCL1/2/8 and performed RNA-Seq assay.

2019-05-17 | GSE131306 | GEO

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