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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of BMDCs treated without or with exosomes from tumor cells or normal cells

ABSTRACT: The goals of this study are to compare the transcriptome profiling (RNA-seq) of tumor cell-derived exosome treated BMDCs to normal cell-derived exosome treaed BMDCs or untreated BMDCs to explore the differential regulated signaling pathways or cellular processes.

ORGANISM(S): Mus musculus

PROVIDER: GSE155881 | GEO | 2021/01/04

REPOSITORIES: GEO

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small RNAseq in donor BMDCs and BMDCs that received exosomes

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2016-07-03 | E-GEOD-67946 | biostudies-arrayexpress

small RNAseq in donor BMDCs and BMDCs that received exosomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Manganese (II) or LPS treated Wild Type BMDC Transcriptomes

Project description:Purpose: The goals of this study are to compare the different functions of manganese (II) and LPS on the activation and maturation of antigen presenting cells-BMDCs. Methods: BMDCs were generated by culturing bone marrow cells with 20 ng/ml of IL-4 (Genscript) and 20 ng/ml of GM-CSF (Genscript) at 37°C and 5% CO2 for 7 days.Then BMDCs were treated with MnCl2 (200 μM) or LPS (100 ng/ml) for 20 h. Then mRNAs were purified, sequenced and analyzed. Results: Using an optimized data analysis workflow, we compared 20339 transcripts in BMDCs. Manganese (II) induced 1677 transcripts expression with a fold change ≥1.5 and LPS induced 1555 transcripts expression with a fold change ≥1.5. Conclusion: Manganese (II) activates the production of type I IFNs and the maturation of BMDCs. But there are also some differences between functions of manganese (II) and LPS on BMDCs.

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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of BMDCs treated without or with exosomes from tumor cells or normal cells

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Ad-MyD88 infected bmDCs: Control vs. Ad-MyD88 infected

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