Effect of 3-deazaadenosine on the H3K36me3 chromatin landscape during senescence [ChIP-seq]

ABSTRACT: We treated IMR90 ER:RAS cells with 4OHT to induce RAS activation or with vehicle (DMSO) as a non-senescent control. At the same time, cells were treated with 10 µM 3-deaazadenosine. 3-deazaadenosine is a SAM-dependent methyltransferase inhibitor. H3K36me3 is a mark enriched at actively transcribed genes. Our previous experiments showed that treatment with 3-deazaadenosine prevents senescence and reduces overall levels of H3K36me3. By performing ChIP-seq, we have unveiled that treatment with 3-deazaadenosine prevents deposition of H3K36me3 in genes essential for the secretory phenotype of senescent cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE155905 | GEO | 2022/07/19

REPOSITORIES: GEO